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US 5,688,684 AGranted
Recombinant .beta.-amylase
AssigneeSapporo Breweries Limited
InventorsNaohiro Yoshigi, Hideo Maeba, Yukio Okada
Life of this patent
Estimating the term…
Abstract
A recombinant .beta.-amylase which is superior to the original recombinant .beta.-amylase in thermostability has been obtained by a site-directed mutagenesis with the recombinant .beta.-amylase gene coding 531 amino acid residues. Substitutions were MET.sub.181 of said enzyme with Leu, Ser.sub.291 with Ala, Ile.sub.293 with Val, Ser.sub.346 with Pro, Ser.sub.347 with Pro, Gln.sub.348 with Asp and Ala.sub.372 with Ser.
Claims6
An isolated gene coding for a recombinant .beta.-amylase having an amino acid sequence denoted by SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.