Process for the preparation of monoterpenes using bacterium containing recombinant DNA
AssigneeBoard of Trustees operating Michigan State University
InventorsPatrick J. Oriel, Srinivasan Savithiry, Hae Choon Chang
Life of this patent
Estimating the term…
Abstract
A process for producing monoterpenes using a DNA segment Bacillus stearothermophilus in a recombinant bacterium, particularly Escherichia coli. The useful monoterpenes produced from limonene are perillyl alcohol, perillyl aldehyde, perillic acid (path A); alpha-terpineol (path B) or carveol and carvone (path C).
Claims36
A recombinant plasmid containing a segment of DNA of a Bacillus stearothermophilus which provides a bacterium with the ability to convert limonene to a monoterpene compound in a culture medium.
A recombinant plasmid as contained in Escherichia coli and deposited as ATCC 69817.
Escherichia coli containing a segment of DNA of segment of DNA of Bacillus stearothermophilus which provides a bacterium with the ability to convert limonene to a monoterpene compound in a culture medium.
A bacterium which is Escherichia coli deposited as ATCC 69817.
A process for producing monoterpene compounds which comprises:
- (a) providing a culture medium containing cells of Escherichia coli containing a segment of DNA of Bacillus stearothermophilus which provides a bacterium with the ability to convert limonene to a monoterpene compound;
- and
- (b) incubating the cells in the culture medium in the presence of the limonene to produce isolatable amounts of the monoterpene compound in the culture medium.
A process for the production of carveol which comprises:
- (a) providing a culture medium containing cells of Escherichia coli containing a segment of DNA of Bacillus stearothermophilus which provides a bacterium with the ability to convert limonene to carveol and carvone in the culture medium.
An Escherichia coli containing a segment of DNA from Bacillus stearothermophilus which converts limonene to carveol and carvone.
A recombinant plasmid containing a segment of DNA from Bacillus stearothermophilus which provides a bacterium with the ability to convert limonene to carveol.
A recombinant plasmid as contained in Escherichia coli deposited as ATCC 69817.
An isolated and purified segment of DNA which is 9.6 kb segment of endonuclease EcoRI digest of DNA of Bacillus stearothermophilus deposited as ATCC 55596.
An isolated and purified segment of DNA which is a 3.8 kb endonuclease HindIII digest of a 9.6 kb segment endonuclease EcoRI digest of DNA of Bacillus stearothermophilus deposited as ATCC 55596.
Dependencies are inferred from the claim text.