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US 5,688,671 AGranted
Mutant aspartokinase gene
AssigneeAjinomoto Co., Inc.
InventorsMasakazu Sugimoto, Yuri Ogawa, Tomoko Suzuki, Akiko Tanaka, Hiroshi Matsui
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AssigneeAjinomoto Co., Inc.
InventorsMasakazu Sugimoto, Yuri Ogawa, Tomoko Suzuki, Akiko Tanaka, Hiroshi Matsui
Estimating the term…
Recombinant DNA replicable in microorganisms belonging to the genus Corynebacterium, which contains a DNA fragment coding for an aspartokinase .alpha.-subunit protein originating from a bacterium belonging to the genus Corynebacterium, in which synergistic feedback inhibition by L-lysine and L-threonine is substantially desensitized, and a DNA fragment coding for an aspartokinase .beta.-subunit protein originating from a bacterium belonging to the genus Corynebacterium, in which synergistic feedback inhibition by L-lysine and L-threonine is substantially desensitized, is introduced into a microorganism belonging to the genus Corynebacterium. Thus a transformant having enhanced production and excretion speeds of L-lysine is obtained. The transformant is cultivated in an appropriate medium, and produced L-lysine is separated.
A recombinant DNA fragment coding for an aspartokinase .alpha.-subunit protein originating from a bacterium belonging to the genus Corynebacterium, in which synergistic feedback inhibition by L-lysine and L-threonine is substantially desensitized, said protein having an amino acid sequence defined by SEQ ID NO:
A recombinant DNA fragment coding for an aspartokinase .beta.-subunit protein originating from a bacterium belonging to the genus Corynebacterium, in which synergistic feedback inhibition by L-lysine and L-threonine is substantially desensitized, said protein having an amino acid sequence defined by SEQ ID NO:
An aspartokinase .alpha.-subunit protein originating from a bacterium belonging to the genus Corynebacterium, in which synergistic feedback inhibition by L-lysine and L-threonine is substantially desensitized, said protein having an amino acid sequence defined by SEQ ID NO:
An aspartokinase .beta.-subunit protein originating from a bacterium belonging to the genus Corynebacterium, in which synergistic feedback inhibition by L-lysine and L-threonine is substantially desensitized, said protein having an amino acid sequence defined by SEQ ID NO:
Dependencies are inferred from the claim text.