Gene encoding carboxypeptidase of Aspergillus niger
AssigneeNovo Nordisk Biotech, Inc.
InventorsDebbie Sue Yaver, Sheryl Ann Thompson
Life of this patent
Estimating the term…
Abstract
The present invention relates to a gene encoding an ascomycete or deuteromycete carboxypeptidase Y gene, and host cells modified so as to produce reduced amounts of carboxypeptidase.
Claims22
A mutant filamentous ascomycete cell which produces at least 25% less carboxypeptidase Y than a corresponding wild-type cell when cultured under identical conditions, wherein the endogenous carboxypeptidase Y gene has been replaced by homologous recombination with a nucleic acid sequence wherein the nucleic acid sequence is selected from the group consisting of (i) the nucleic acid sequence depicted in SEQ ID NO:1 or SEQ ID NO:3 and (ii) a nucleic acid sequence which hybridizes with SEQ ID NO:1 or SEQ ID NO:3 under high stringency conditions;
- which sequence has been disrupted;
- and which cell has been transformed to recombinantly produce a heterologous protein.
Dependencies are inferred from the claim text.