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AssigneeUnited States of America
InventorsTimothy J. Lott, Christine J. Morrison, Errol Reiss, Brent Lasker, Sandra Zakroff
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Provided is an isolated double-stranded nucleic acid consisting essentially of the nucleotide sequences defined in the Sequence Listing by SEQ ID Nos:5-9. These are the ITS2 sequences for C. albicans, C. parapsilosis, C. tropicalis, C. glabrata and C. krusei. A method of diagnosing systemic candidiasis in a subject is also provided. The method comprises the steps of: (a) collecting blood from the subject into tubes containing detergent, polypropylene glycol, sodium poyantholesulfonate, and sodium ethylene diamine tetraacetic acid; (b) lysing Candida cells using ZYMOLYASE.RTM.-100T.TM. enzyme mixture with agitation; (c) extracting and precipitating the DNA from the lysed cells; (d) amplifying the precipitated DNA using universal fungal primer pairs derived from the internal transcribed spacer regions of the Candida ribosomal DNA; and (e) detecting amplified DNA from Candida by hybridizing the amplified DNA with a probe that selectively hybridizes with Candida DNA, the presence of amplified DNA indicating systemic candidiasis.
An isolated double stranded nucleic acid consisting of the nucleotide sequence of SEQ ID NO:6.
An isolated nucleic acid, separated from other nucleic acids found in a naturally occurring organism, that is complementary to and specifically hybridizes with the nucleic acid of claim 3.
A method of diagnosing systemic candidiasis in a subject comprising the steps of:
A method of diagnosing systemic candidiasis in a subject comprising the steps of:
An isolated double stranded nucleic acid selected from the group consisting of the nucleotide sequence of SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, and SEQ ID NO:
A method for diagnosing systemic candidiasis in a patient comprising hybridizing DNA released from lysed Candida cells present in a blood sample of the patient with a probe that is complementary to and specifically hybridizes with a nucleic acid selected from the group consisting of the nucleic acid of SEQ ID NO:5, the nucleic acid of SEQ ID NO:6, the nucleic acid of SEQ ID NO:7, the nucleic acid of SEQ ID NO:8, and the nucleic acid of SEQ ID NO:9;
Dependencies are inferred from the claim text.