Detection of isoniazid resistent strains of M. tuberculosis
AssigneeMayo Foundation for Medical Education and Research
InventorsFranklin R. Cockerill, Bruce C. Kline, James R. Uhl
Life of this patent
Estimating the term…
Abstract
A method for determining the susceptibility of a strain of M. tuberculosis to isoniazid is provided comprising employing the techniques of restriction length polymorphism analysis to determine whether or not the DNA of said strain has an NciI-MspI restriction site at the codon corresponding to codon 463 of an M. tuberculosis katG gene consensus sequence.
Claims7
A method for determining the susceptibility of a strain of M. tuberculosis to isoniazid comprising employing the technique of restriction length polymorphism analysis to determine whether a NciI-MspI restriction site is absent in the DNA of said strain at the codon corresponding to codon 463 of the M. tuberculosis katG gene consensus sequence depicted in FIG. 1 (SEQ ID NO:1), wherein said absence is indicative of an INH-resistant strain.
An oligonuclcotidc selected from the group consisting of SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:
- 5, SEQ ID NO:6, and subunits thereof which subunits are effective for the amplification of a region incorporating codon 463 of M. tuberculosis katG gene.
Dependencies are inferred from the claim text.