Back to results
US 5,688,637 AGranted
Nucleotide sequences derived from the genome of retroviruses of the HIV-1, HIV-2 and SIV type, and their uses in particular for the amplification of the genomes of these retroviruses and for the in vitro diagnosis of the disease due to these viruses
AssigneesInstitut Pasteur, Institut National de la Sante et de la Recherche Medicale
InventorsMaurice Moncany, Luc Montagnier
Life of this patent
Estimating the term…
Abstract
The invention relates to nucleotidic sequences derived from genomes of the HIV-1 type virus, or from genomes of the HIV-2 type virus, or of the SIV type virus, and their applications, especially as oligo-nucleotidic initiators of implementation of an $i (in vitro) method for the diagnosis of the infection of an individual by a virus of the HIV-1 and/or HIV-2 type.
Claims14
An oligonucleotide primer, said primer having a nucleotide sequence selected from the following group of nucleotides oriented in the 5'-3' direction:
- nucleotides 636-653, 854-872, 1369-1388, and 2021-2039 of the gag gene of HIV-1 Bru;
- nucleotides 900-881, 1385-1369, 1388-1369, and 2039-2021 of a nucleic acid sequence complementary to the gag gene of HIV-1 Bru;
- nucleotides 635-652, 864-888, 1403-1421, and 2055-2073 of the gag gene of HIV-1 Mal;
- nucleotides 916-897, 1419-1403, 1421-1403, and 2073-2055 of a nucleic acid sequence complementary to the gag gene of HIV-1 Mal;
- nucleotides 636-653, 848-872, 1369-1388, and 2024-2042 of the gag gene of HIV-1 Eli;
- nucleotides 900-881, 1385-1369, 1388-1369, and 2042-2024 of a nucleic acid sequence complementary to the gag gene of HIV-1 Eli;
- nucleotides 859-876, 1160-1184, 1687-1706, and 2329-2349 of the gag gene of HIV-2 ROD;
- nucleotides 1212-1193, 1703-1687, 1706-1687, and 2349-2329 of a nucleic acid sequence complementary to the gag gene of HIV-2 ROD;
- nucleotides 834-851, 1124-1148, 1651-1670, and 2299-2318 of the gag gene of SIV-MAC;
- and
- nucleotides 1176-1157, 1667-1651, 1670-1651, and 2318-2299 of a nucleic acid sequence complementary to the gag gene of SIV-MAC;
- nucleotides 5590-5610 of the vpr gene of HIV-1 Bru;
- nucleotides 5870-5849 of a nucleic acid sequence complementary to the vpr gene of HIV-1 Bru;
- nucleotides 5585-5605 of the vpr gene of HIV-1 Mal;
- nucleotides 5865-5844 of a nucleic acid sequence complementary to the vpr gene of HIV-1 Mal,
- nucleotides 5554-5574 of the vpr gene of HIV-1 Eli;
- nucleotides 5834-5813 of a nucleic acid sequence complementary to the vpr gene of HIV-1 Eli;
- nucleotides 6233-6296 of the vpr gene of HIV-2 ROD;
- nucleotides 6551-6531 of a nucleic acid sequence complementary to the vpr gene of HIV-2 ROD;
- nucleotides 6147-6170 of the vpr gene of SIV-MAC;
- and
- nucleotides 6454-6431 of a nucleic acid sequence complementary to the vpr gene of SIV-MAC;
- nucleotides 2620-2643, 3339-3361, 4186-4207, and 4992-5011 of the pol gene of HIV-1 Bru;
- nucleotides 2643-2620, 3361-3339, 4207-4186, and 5011-4992 of a nucleic acid sequence complementary to the pol gene of HIV-1 Bru;
- nucleotides 2615-2638, 3333-3356, 4181-4202, and 4987-5006 of the pol gene of HIV-1 Mal;
- nucleotides 2638-2615, 3356-3334, 4202-4181, and 5006-4987 of a nucleic acid sequence complementary to the pol gene of HIV-1 Mal;
- nucleotides 2584-2607, 3303-3325, 4150-4171, and 4956-4975 of the pol gene of HIV-1 Eli;
- nucleotides 2607-2584, 3325-3303, 4171-4150, and 4975-4956 of a nucleic acid sequence complementary to the pol gene of HIV-1 Eli;
- nucleotides 2971-2994, 3690-3712, 4534-4555, and 5340-5359 of the pol gene of HIV-2 ROD;
- nucleotides 2994-2971, 3712-3690, 4555-4534, and 5359-5340 of a nucleic acid sequence complementary to the pol gene of HIV-2 ROD;
- nucleotides 2887-3010, 3606-3628, 4450-4471, and 5275-5256 of a nucleic acid sequence complementory to the pol gene of SIV-MAC;
- 5256-5275 of the pol gene of SIV-MAC;
- and
- nucleotides 3010-2887, 3628-3606, 4471-4450, and
- nucleotides 9165-9185 and 9542-9564 of the nef2 gene of HIV-2 ROD;
- 9564-9542 and 9956-9933 of a nucleic acid sequence complementary to the nef2 gene of HIV-2 ROD;
- nucleotides 9139-9159 and 9516-9538 of the nef2 gene of SIV-MAC;
- 9538-9516 and 9839-9870 of a nucleic acid sequence complementary to the nef2 gene of SIV-MAC;
- nucleotides 5424-5450 and 5754-5775 of the vif2 gene of HIV-2 ROD;
- nucleotides 5775-5754 and 6082-6061 of a nucleic acid sequence complementary to the vif2 gene of HIV-2 ROD;
- nucleotides 5340-5366 and 5670-5691 of the vif2 gene of HIV-2 ROD;
- nucleotides 5691-5670 and 5995-5974 of a nucleic acid sequence complementary to the vif2 gene of SIV-MAC;
- nucleotides 5900-5918 of the vpx gene of HIV-2 ROD;
- nucleotides 6228-6208 of a nucleic acid sequence complementary to the vpx gene of HIV-2 ROD;
- nucleotides 5813-5831 of the vpx gene of HIV-2 ROD;
- nucleotides 6141-6121 of a nucleic acid sequence complementary to the vpx gene of SIV-MAC;
- nucleotides 6905-6930, 7055-7077, 7360-7384, 7832-7857, 8844-8869, 7629-7647, and 8224-8242 of the env gene of HIV-1 Bru;
- nucleotides 6930-6905, 7384-7360, 7857-7832, 8869-8844, and 8242-8224 of a nucleic acid sequence complementary to the env gene of HIV-1 Bru;
- nucleotides 6903-6928, 7053-7075, 7821-7846, 7821-7846, 7612-7630, 8213-8231, and 8836-8861 of the env gene of HIV-1 Mal;
- nucleotides 6928-6903, 7373-7349, 7846-7821, 8861-8836, and 8231-8213 of a nucleic acid sequence complementary to the env gene of HIV-1 Mal;
- nucleotides 6860-6885, 7010-7032, 7306-7330, 7775-7800, 8787-8812, 7572-7590, and 8167-8185 of the env gene of HIV-1 Eli;
- and
- nucleotides 6885-6860, 7330-7306, 7800-7775, 8812-8787, and 8185-8167 of a nucleic acid sequence complementary to the env gene of HIV-1 Eli;
- nucleotides 9116-9136 of the nef1 gene of HIV-1 Bru;
- nucleotides 9136-9116 and 9503-9483 of a nucleic acid sequence complementary to the nef1 gene of HIV-1 Bru;
- nucleotides 9117-9137 of the nef1 gene of HIV-1 Mal;
- and
- nucleotides 9137-9117 and 9505-9484 of a nucleic acid sequence complementary to the nef1 gene of HIV-1 Mal;
- nucleotides 9062-9082 of the nef1 gene of HIV-Eli;
- nucleotides 9082-9062 and 9449-9428 of a nucleic acid sequence complementary to the nef1 gene of HIV-1 Eli;
- nucleotides 5073-5099 and 5383-5405 of the vif1 gene of HIV-1 Bru;
- and
- nucleotides 5405-5383 and 5675-5653 of a nucleic acid sequence complementary to the vif1 gene of HIV-1 Bru;
- nucleotides 5068-5094 and 5378-5400 of the vif1 gene of HIV-1 Mal;
- nucleotides 5400-5378 and 5670-5648 of a nucleic acid sequence complementary to the vif1 gene of HIV-1 Mal;
- and
- nucleotides 5037-5063 and 5347-5369 of the vif1 gene of HIV-1 Eli;
- nucleotides 5369-5347 and 5639-5617 of a nucleic acid sequence complementary to the vif1 gene of HIV-1 Eli;
- nucleotides 6081-6105 and 6240-6263 of the vpu gene of HIV-1 Bru;
- nucleotides 6343-6321 of a nucleic acid sequence complementary to the vpu gene of HIV-1 Bru;
- nucleotides 6076-6100 and 6238-6261 of the vpu gene of HIV-1 Mal;
- nucleotides 6338-6316 of a nucleic acid sequence complementary to the vpu gene of HIV-1 Mal;
- nucleotides 6045-6069 and 6207-6230 of the vpu gene of SIV-MAC;
- and
- nucleotides 6307-6285 of a nucleic acid sequence complementary to the vpu gene of SIV-MAC.
72 more elements
An oligonucleotide primer selected from the group consisting of primers having the following nucleotide sequences from 5' to 3':
- MMy1:
- TGG CGC CCGAAC AGG GAC
- TGG CGC CTGAAC AGG GAC
- MMy2:
- GGC CAG GGG GAAAGAAAAA
- GGC CCG GCG GAAAGAAAAA
- MMy3:
- TGC CCA TACAAAATG TTT TA
- TGC CCA CAC TAT ATG TTT TA
- MMy4:
- TGC ATG GCT GCT TGA TG
- TGC ATA GCT GCC TGG TG
- MMy4B:
- CTT TGC ATG GCT GCT TGA TG
- CTC TGC ATA GCT GCT TGC TG
- MMy4Ba:
- CAT CAAGCA GCC ATG CAAAG
- CAC CAG GCA GCT ATG CAG AG
- MMy28:
- AGG GCT GTT GGAAAT GTG G
- AGG GCT GTT GGA AGT GTG G
- MMy28a:
- CCA CAT TTC CAG CAT CCC T
- CCA CAT TTC CAG CAG CCC T
- CCA CAT TTC CAG CAC CCC T
- MMy18:
- GAT AGA TGGAAC AAG CCC CAG
- MMy19:
- TCC ATT TCT TGC TCT CCT CTG T
- MMy29:
- TAAAGC CAG GAA TGG ATG GCC CAA
- TAAAGC CAG GAA TGG ATG GAC CAA
- MMy29a:
- TTG GGC CAT CCA TTC CTG GCT TTA
- TTG GTC CAT CCA TTC CTG GCT TTA
- MMy30:
- TGG ACT GTC AAT GAC ATA CAGAA
- TGG ACT GTC AAT GAT ATA CAGAA
- MMy30a:
- TTC TGT ATG TCA TTG ACA GTC CA
- TTC TGT ATG TCA TTG ACT GTC CA
- MMy31:
- CAT GGG TAC CAG CAC ACAAAG G
- MMy31a:
- CCT TTG TGT GCT GGT ACC CAT G
- MMy32:
- TGG AAA GGT GAA GGG GCA GT
- TGG AAA GGT GAAGGA GCA GT
- MMy32a:
- ACT GCC CCT TCA CCT TTC CA
- ACT GCC CCT TCT CCT TTC CA
- ACT GCC CCT TCC CCT TTC CA
- MMy12:
- AGA GAC TCT TGC GGG CGC GTG
- MMy13:
- ATA TAC TTA GAAAAG GAA GAAGG
- MMy13a:
- CCT TCT TCC TTT TCTAAG TAT AT
- MMy14:
- AGC TGA GAC AGC AGG GAC TTT CCA
- MMy20:
- TAT GGA GGA GGAAAAGAG ATG GAT AGT
- MMy21:
- TAG CAC TTA TTT CCC TTG CTT T
- MMy21a:
- AAA GCA AGG GAAATA AGT GCT A
- MMY22:
- CCC TTG TTC ATC ATG CCA GTA T
- MMy23:
- ATG TCA GAT CCC AGG GAG A
- MMy24:
- CCT GGA GGG GGA GGA GGA GGA
- MMy5:
- CCA ATT CCC ATA CAT TAT TGT GCC CC
- MMy5a:
- GGG GCA CAA TAATGT ATG GGA ATT GG
- MMy6:
- AAT GGC AGT CTA GCA GAA GAA GA
- MMy7:
- ATC CTC A0G AGG GGA CCC AGAAAT T
- MMy7a:
- AAT TTC TGG GTC CCC TCC TGA GGA T
- MMy8a:
- GTG CTT CCT GCT GCT CCC AAG AAC CC
- MMy8a:
- GGG TTC TTG GGA GCA GCA GGA AGC AC
- MMy9:
- ATG GGT GGC AAG TGG TCAAAAAGT AG
- ATG GGT GGCAAATGG TCAAAAAGT AG
- MMy9a:
- CTA CTT TTT GAC CAC TTG CCA CCC AT
- MMy89:
- TTC ATT CTT TTC TTG CTG G
- MMy10:
- AAAAGAAAAGGG GGG ACT GGA
- MMy10a:
- TCC AGT CCC CCC TTT TCT TTT
- MMy11:
- AAA GTC CCC AGC GGAAAG TCC C
- MMy15:
- GAT TAT GGAAAA CAG ATG GCA GGT GAT
- MMy16:
- GCAGAC CAACTA ATT CAT CTG TA
- MMy16a:
- TAC AGA TGA ATT AGT TGG TCT GC
- MMy17:
- CTT AAG CTC CTC TAAAAG CTC TA
- MMy25:
- GTA AGT AGT ACA TGTAAT GCA ACC T
- MMy26:
- AGC AGA AGA CAG TGG CCATGA GAG
- MMy27:
- ACT ACA GAT CAT CAATAT CCC AA.
110 more elements
Dependencies are inferred from the claim text.