Synthesis, deprotection, analysis and purification of RNA and ribozymes
AssigneeRibozyme Pharmaceuticals, Inc.
InventorDanuta Tracz
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Abstract
Method for purification and synthesis of RNA molecules and enzymatic RNA molecules in enzymatically active form.
Claims3
Method for one pot deprotection of RNA comprising, contacting a protected base with anhydrous methyl amine at between 60.degree. C.-70.degree. C. for at least 5 min, cooling the resulting mixture and contacting said mixture with TEA-3HF reagents under conditions which remove a protecting group of the 2'-hydroxyl position.
Method for one pot deprotection of RNA comprising, contacting a protected base with anhydrous ethyl amine at between 60.degree. C.-70.degree. C. for at least 5 min, cooling the resulting mixture and contacting said mixture with TEA-3HF reagents under conditions which remove a protecting group of the 2'-hydroxyl position.
Dependencies are inferred from the claim text.