Domains of extracellular region of human platelet derived growth factor receptor polypeptides
AssigneesCOR Therapeutics, Inc., The Regents of the University of California
InventorsDavid Wolf, James E. Tomlinson, Larry J. Fretto, Neill A. Giese, Jaime A. Escobedo, Lewis Thomas Williams
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Abstract
Defined constructs of modified human platelet-derived growth factor receptor polypeptides are provided. Extracellular region domain structures are identified and modifications and combinatorial rearrangements of the receptor segments are provided. Both cell bound and soluble forms of modified segments are made available, as are methods for assays using them, allowing for screening of ligand analogues.
Claims8
A type B or a type A human platelet-derived growth factor receptor (hPDGF-R) fragment consisting of one or two extracellular domains, said domains selected from the group consisting of one or two of only D1, D2, and D3, said fragment having platelet-derived growth factor receptor ligand binding activity, wherein said fragment binds a platelet-derived growth factor ligand with a K.sub.D of less than about 10 .mu.M.
A hPDGF-R fragment consisting of the intra-cysteine portion of domain D3.
A type B or a type A hPDGF-R fragment wherein said fragment consists of extracellular domains D1 and D2, and wherein said fragment has platelet-derived growth factor receptor ligand binding activity, and binds a platelet-derived growth factor ligand with a K.sub.D of less than about 10 .mu.M.
Dependencies are inferred from the claim text.