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US 5,686,315 AGranted
Assay device for one step detection of analyte
AssigneeQuidel Corporation
InventorsAllan D. Pronovost, Cathy A. Bacquet, Jan W. Pawlak, Theodore T. Sand
Life of this patent
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Abstract
A "one step" device for the detection of analytein clinical assays is disclosed. A visible label comprising a visible moiety and a ligand that binds to or competes with analyte is contained in a fluid conducting membrane or prefilter; and the analyte and associated ligand are conducted by the flow of sample into a detection zone. The detection zone contains a capture reagent that binds to label or to analyte bound to label.
Claims15
An assay device for one-step detection of the presence or absence of an analyte in a sample, wherein said device comprises:
- a) a removable fluid-conducting membrane, to which the sample is applied, containing a mobilizable label, which label comprises a visible moiety coupled to a ligand which ligand specifically binds said analyte or competes with said analyte for a specific binding partner to the analyte, said membrane being in fluid communication with and on top of
- b) a matrix containing a first detection zone wherein said detection zone contains immobilized thereon said specific binding partner to the analyte, said matrix being in fluid communication with and on top of
- c) an absorbent capable of drawing liquids applied to said device through said membrane and said matrix.
Dependencies are inferred from the claim text.