Establishment of new cell lines from Pseudaletia unipuncta with differential responses to baculovirus
AssigneeBoyce Thompson Institute for Plant Research, Inc.
InventorsPing Wang, Robert R. Granados
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Abstract
This disclosure presents the establishment of a new cell line from Pseudaletia unipuncta embryos. This cell line demonstrated the ability to produce high numbers of baculoviruses in cell culture. These virus particles are found internally in the cells in occlusion bodies. In the study of Pseudaletia unipuncta two baculoviruses were found to infect this species: P. unipuncta nuclear polyhedrosis virus (PuNPV), and P. unipuncta granulosis virus (PuGV). In addition, the cell line was also selected and cultured for its ability to grow in suspension while maintaining high levels of OB production.
Claims7
An insect cell line established from embryonic egg cells from an insect from the order Lepidoptera which is designated BTI-Pu-527A7S and has been deposited under the accession number ATCC CRL 12285 and has the following characteristics:
- a) supports replication of virus,
- b) supports expression of protein after infection by a recombinant virus in a serum containing medium,
- c) can grow in suspension and/or shaker flask cell cultures;
- and
- d) grows in said serum containing medium and retains said ability to support replication of virus and to support expression of protein.
An insect cell line with all of the identifying characteristics of the cell line identified as BTI-Pu-527A7S and deposited under the accession number ATCC CRL 12285.
An insect cell line established from the embryonic egg cells of the insect Pseudaletia unipuncta, from the order Lepidoptera, which is designated BTI-Pu-527A75 having all of the identifying characteristics of the insect cell line.
Dependencies are inferred from the claim text.