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AssigneeToyo Boseki Kabushiki Kaisha
InventorsAtsushi Sogabe, Seiji Takeshima, Kazumi Yamamoto, Shinichi Teshima, Shigenori Emi, Yoshihisa Kawamura
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A novel protein having a heat-resistant malate dehydrogenase activity, a DNA fragment having a gene encoding said protein, a recombinant vector having said DNA fragment, a transformant transformed with said vector, a method for producing the protein having heat-resistant malate dehydrogenase activity by the use of said transformant, a reagent for GOT determination, comprising the above-mentioned novel protein having a heat-resistant malate dehydrogenase activity and a method for determining GOT activity, which comprises the use of said reagent. According to the present invention, a protein having a heat-resistant malate dehydrogenase activity and having higher purity and superior heat stability can be obtained. In addition, a reagent for GOT determination which is superior in long-term storage can be prepared by the use of the protein having a heat-resistant malate dehydrogenase activity.
An isolated DNA fragment having a nucleotide sequence encoding the amino acid sequence depicted in SEQ ID NO:2.
An isolated DNA fragment having a nucleotide sequence depicted in SEQ ID NO:1.
Dependencies are inferred from the claim text.