Marek's disease virus vaccine
AssigneeAkzo Nobel N.V.
InventorWilliam Baxendale
Life of this patent
Estimating the term…
Abstract
The present invention is concerned with a new strain of Mareks Disease Virus and to a vaccine for the protection of poultry against Mareks Disease containing the novel strain. The invention also relates to bivalent or polyvalent vaccines comprising in addition other viruses of the Mareks Disease virus group, i.e. HVT.
Claims18
An attenuated strain of Marek's Disease Virus serotype I which, when liberated from infected cells by sonication, results in at most a 100-fold decrease in cell-free virus titre compared with the cell-associated virus titre.
An attenuated cell-free strain of Marek's Disease Virus serotype I which is deposited at the European Collection of Animal Cell Cultures, Porton Down, UK under accession No. V94062211.
A method for the preparation of a vaccine that protects poultry against Marek's Disease which comprises:
- (a) growing a serotype I Marek's Disease virus in cell culture from which sufficient quantities of cell-free virus necessary to prepare an effective immunizing dosage can be obtained,
- (b) disrupting the cells,
- (c) subsequently collecting the cell-free viruses, and
- (d) subjecting the material obtained from step (c) to at least one of the following treatments:
- i clarifying by centrifugation and/or filtration;
- ii adding buffer;
- iii adding a stabilizing agent;
- iv putting the material in a vial;
- v freeze-drying.
Dependencies are inferred from the claim text.