Genomic DNA encoding the pseudomonas fluorescens alternative sigma factor, RpoS, capable of activating gene expression
AssigneeNovartis Finance Corporation
InventorsThomas D. Gaffney, Stephen T. Lam
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Abstract
Gene activating sequences which activate the expression of other bacterial genes, which are latent or expressed at low levels, are provided. The gene activating sequences confer the ability to produce several metabolites and may be transferred to bacterial strains. The transformed biocontrol agents are active to inhibit the growth of the fungal pathogens.
Claims16
An isolated gene activating element comprising a nucleotide sequence capable of inducing the expression of at least one gene in a Pseudomonad selected for transformation, wherein said gene is latent or expressed at low levels in said Pseudomonad, and wherein said nucleotide sequence is an rpoS sequence isolated from Pseudomonas fluorescens.
An isolated DNA molecule that encodes a sigma factor having an amino acid sequence defined by that set forth in SEQ ID NO:
- 9.
A method for activating expression in a Pseudomonas host of at least one gene that is latent or natively expressed at low levels, comprising introducing into said host at least one copy of a DNA molecule that encodes a Pseudomonas RpoS sigma factor.
Dependencies are inferred from the claim text.