Methods for enhanced retrovirus-mediated gene transfer
AssigneeIndiana University Foundation
InventorsDavid A. Williams, Vikram P. Patel
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Abstract
A method to increase the efficiency of transduction of hematopoietic cells by retroviruses includes infecting the cells in the presence of fibronectin or fibronectin fragments. The fibronectin and fibronectin fragments significantly enhance retroviral-mediated gene transfer into the hematopoietic cells, particularly including committed progenitors and primitive hematopoietic stem cells. The invention also provides improved methods for somatic gene therapy capitalizing on the enhanced gene transfer, and hematopoietic cellular populations.
Claims22
A method for increasing the frequency of transduction of hematopoietic cells by a replication-defective recombinant retrovirus vector, comprising infecting hematopoietic cells with a replication-defective recombinant retrovirus vector in the presence of substantially pure fibronectin, substantially pure fibronectin fragments, or a mixture thereof, so as to increase the frequency of transduction of the hematopoietic cells by the retrovirus vector.
A method for producing transduced hematopoietic cells, comprising:
- infecting hematopoietic cells in culture with a replication-defective recombinant retrovirus in the presence of immobilized fibronectin, immobilized fibronectin fragments, or an immobilized mixture thereof, to produce transduced hematopoietic cells.
A method for improving retroviral-mediated gene transfer in hematopoietic cells, comprising conducting the retroviral-mediated gene transfer in the presence of immobilized fibronectin, immobilized fibronectin fragments or an immobilized mixture thereof.
A composition comprising:
- a viable hematopoietic cellular population transduced by retroviral-mediated gene transfer;
- and
- immobilized fibronectin, immobilized fibronectin fragments, or an immobilized mixture thereof, in the presence of which said population has been transduced by the retroviral-mediated gene transfer;
- said composition being free from virus-producing cells.
Dependencies are inferred from the claim text.