Bioconversion of a fermentable carbon source to 1,3-propanediol by a single microorganism
AssigneeE. I. Du Pont de Nemours and Company
InventorsLisa Anne Laffend, Vasantha Nagarajan, Charles Edwin Nakamura
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Abstract
A process is provided for the bioconversion of a carbon substrate to 1,3-propanediol by a single organism utilizing either microorganisms containing the genes encoding for an active glycerol or diol dehydratase enzyme by contacting these organisms with a carbon substrate under the appropriate fermentation conditions.
Claims16
A process comprising the bioconversion of a carbon substrate, other than glycerol or dihydroxyacetone, to 1,3-propanediol by a single microorganism having at least one gene that expresses a dehydratase enzyme by contacting said microorganism with said substrate.
A process for the bioconversion of a carbon substrate to 1,3-propanediol by a single microorganism comprising:
- (i) contacting a medium containing at least one carbon substrate with a single microorganism to yield a culture medium, wherein the at least one carbon substrate is selected from the group consisting of monosaccharides, oligosaccharides, and polysaccharides, provided that the carbon substrate is other than glycerol or dihydroxyacetone, and wherein said single microorganism is selected from the group consisting of members of the genera Klebsiella, Citrobacter, recombinant Escherichia, or is a recombinant organism transformed with a gene encoding a diol dehydratase enzyme or a glycerol dehydratase enzyme,
- (ii) incubating said culture medium under suitable conditions to produce 1,3-propanediol;
- and
- (iii) recovering said 1,3-propanediol.
Dependencies are inferred from the claim text.