Synthesis of homochiral 2-hydroxy acids
AssigneeGenzyme Corporation
InventorsGuy Casey, Thomas Lee, deceased, Victor Lee, administrator, Eileen Ann Lee, administratrix
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Abstract
A process for the production of a homochiral 2-hydroxy carboxylic acid or salt thereof corresponding to general formula (I), wherein R represents one of formulae (II), (III), (IV), (V), wherein R' represents straight- or branched-chain alkyl or phenyl optionally para-substituted with methyl, methoxy, nitro or amino; and R" represents hydrogen, halogen or phenyl optionally para-substituted with methyl, methoxy, nitro or amino; and M represents hydrogen or a salt-forming moiety; characterized in that it comprises reducing a corresponding 2-keto carboxylic acid or salt thereof using a (R)- or (S)-2-oxo carboxylic acid dehydrogenase, inter alia, is disclosed.
Claims6
A process for the production of a homochiral 2-hydroxy carboxylic acid or salt thereof corresponding to the following general formula:
- ##STR53## wherein R represents one of the following:
- ##STR54## wherein R' represents phenyl optionally para-substituted with methyl, methoxy, nitro or amino;
- and R" represents hydrogen, halogen or phenyl optionally parasubstituted with methyl, methoxy, nitro or amino;
- and
- M represents hydrogen or a salt-forming moiety;
- characterized in that it comprises reducing a corresponding 2-keto carboxylic acid or salt thereof using a (R)- or (S)-lactate dehydrogenase obtained from Bacillus stearothermophilus or Staphylococcus epidermis.
Dependencies are inferred from the claim text.