Leukocyte adhesion receptors
AssigneeDana-Farber Cancer Institute, Inc.
InventorsAngel A. Corbi, Timothy A. Springer
Life of this patent
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Abstract
This invention concerns the cloning of the alpha-subunit of the p150,95 leukocyte adhesion receptor molecule. The invention also concerns the alpha-subunit of p150,95, as well as fragments of this receptor molecule. The invention further pertains to the diagnostic and therapeutic uses for such molecules.
Claims11
A polypeptide, substantially free of natural contaminants, wherein said peptide is the alpha-subunit of p150,95 having an absolute molecular weight of about 126,000 kD and having the amino acid sequence set forth in FIG. 3, or naturally occurring allelic or species variants thereof.
A soluble polypeptide substantially free of natural contaminants, wherein said polypeptide is a solubilized form of alpha-subunit of p150,95, wherein said alpha-subunit of p150,95 has an amino acid sequence of FIG. 3.
A process of recombinantly producing the alpha-subunit of human p150,95 comprising:
- a) providing an isolated nucleic acid molecule comprising a sequence which encodes the alpha-subunit of human p150,95, wherein said human p150,95 has the amino acid sequence depicted in FIG. 3;
- b) transforming a host cell with a vector comprising said isolated nucleic acid molecule, wherein said vector is capable of expressing the alpha-subunit of human p150,95 in said host cell;
- and
- c) culturing said transformed host cell so as to express the alpha-subunit of human p150,95.
Dependencies are inferred from the claim text.