Compositions and methods relating to transdominant Tat mutants
AssigneeBoard of Regents, The University of Texas Sys
InventorsRichard B. Gaynor, Joseph A. Garcia, David Harrich
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Abstract
Transdominant HIV tat substitution and truncated gene mutants of 72 amino acid residues or less are disclosed. The mutated genes encode mutant Tat proteins which are capable of inhibiting the expression of the HIV-1 virus in the presence of an equimolar concentration of the wild type Tat protein in vitro. Therapeutic agents which include fused protein forms of the mutant proteins are also disclosed, as well as methods of preparing and using the therapeutic agents in the treatment of HIV infection and HIV-related injections in an animal. Recombinant vectors which express the mutant HIV Tat proteins described are also disclosed, as well as cell lines which product high yields of the mutant HIV.
Claims21
A recombinant vector comprising a DNA sequence encoding a transdominant mutant HIV Tat protein that inhibits HIV gene expression in the presence of an equimolar concentration of wild-type Tat protein, wherein the DNA sequence is a mutant tat gene having a substituted basic domain in which more than two positively-charged amino acids are replaced by neutral amino acids.
A recombinant vector comprising a DNA sequence encoding a transdominant mutant HIV Tat protein that inhibits HIV gene expression in the presence of an equimolar concentration of wild-type Tat protein wherein the DNA sequence is a mutant tat gone having a substituted basic domain in which more than two positively-charged amino acids are replaced by neutral amino acids, and wherein said vector has a drug resistance gone encoding resistance to puromycin or G418.
A recombinant host bearing a recombinant vector that expresses a transdominant mutant HIV Tat protein that inhibits HIV gene expression in the presence of an equimolar concentration of wild-type Tat protein.
A cell infected with HIV and containing a recombinant vector expressing a transdominant mutant HIV Tat protein, the protein inhibiting HIV gene expression in the presence of ah equimolar concentration of wild-type Tat protein.
A cell infected with HIV and containing a transdominant mutant HIV Tat protein, the protein inhibiting HIV gene expression in the presence of an equimolar concentration of wild-type Tat protein.
Dependencies are inferred from the claim text.