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US 5,686,259 AGranted
Assay method for the detection of 26kd TNF prohormone
AssigneeChiron Corporation
InventorsMichael Kriegler, Carl Perez
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Abstract
Cleavage site blocking antibody that binds to prohormones, preferable Tumor Necrosis Factor, thereby preventing the formation of prohormone fragment(s) by proteolysis of the prohormone, and uses of the antibody including prophylactic and therapeutic methods to treat disease, and diagnostic assays for determining the amount of the prohormone and prohormone fragments present in a patients body.
Claims7
A method for assaying for 26 kd TNF prohormone in the presence of TNF mature hormone in a fluid sample, comprising the steps of:
- a) contacting a sample suspected of having 26 kd TNF prohormone with an immobilized first monoclonal antibody, said immobilized first monoclonal antibody binding to TNF prohormone and forming a first complex therewith, said first monoclonal antibody being substantially non-bindable to said 17 kd TNF mature hormone;
- b) contacting said first complex with a labeled second monoclonal antibody, said second monoclonal antibody capable of binding to TNF mature hormone and further forming a second complex upon binding to the 26 kd prohormone of said first complex;
- and
- c) determining the amount of label in said second complex, whereby the amount of label in said second complex is proportional to the amount of 26 kd TNF prohormone in said sample.
1 more element
Dependencies are inferred from the claim text.