Carbohydrate-based vaccine and diagnostic reagent for trichinosis
AssigneeColorado State University Research Foundation
InventorsNancy Wisnewski, Robert B. Grieve, Donald L. Wassom, Michael R. McNeil
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Abstract
The present invention relates to Trichinella diagnostic reagents that include at least one tyvelose-containing oligosaccharide, or functional equivalent thereof, and use of such reagents to detect Trichinella, and particularly Trichinella spiralis infections. The present invention also includes diagnostic kits based on such reagents and anti-Trichinella spiralis drugs based on the knowledge that tyvelose is produced by Trichinella spiralis parasites.
Claims17
A method to determine Trichinella infection in an animal comprising:
- (a) applying serum collected from said animal onto a surface coated with a diagnostic reagent comprising tyvelose joined through a glycosidic linkage to at least one monosaccharide to form a disaccharide or an oligosaccharide said step of applying being conducted under conditions such that selective binding of an antibody from said serum indicative of Trichinella infection to said reagent-coated surface is accomplished to form a selective binding complex on said reagent-coated surface, said reagent comprising at least one tyvelose terminal residue;
- (b) removing non-bound serum material under conditions that retain said selective binding complex on said reagent-coated surface;
- and
- (c) determining Trichinella infection by detecting said selective binding complex.
A diagnostic kit for detecting Trichinella infection in an animal comprising a diagnostic reagent comprising tyvelose joined through glycosidic linkage to at least one monosaccharide to form a disaccharide or an oligosaccharide, said tyvelose-containing reagent comprising at least one tyvelose terminal residue, said tyvelose being joined to a monosaccharide other than mannose.
Dependencies are inferred from the claim text.