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US 5,686,252 AGranted
Immunoassay method utilizing zeta potential and immunoassay kit
AssigneeHoechst Japan Limited
InventorHiroshi Nishizaki
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Abstract
An immunochemical method is provided for the detection and determination of an analyte. The zeta potential of a latex-particle loaded with an immunologically active substance is measured before and after bringing the loaded latex-particle into contact with an analyte. The difference in zeta potential is correlated with changes of zeta potential for known concentrations of the analyte in order to determine the presence and amount of the analyte.
Claims2
An immunochemical method for the detection and determination of an analyte comprising the steps of:
- a) measuring the zeta potential of a latex-particle loaded with an immunologically active substance being capable of specifically reacting with the analyte suspended in an aqueous electrolyte solution;
- b) bringing into contact the latex-particle loaded with an immunologically active substance with the analyte;
- c) measuring the zeta potential of the latex-particle loaded with an immunologically active substance to which the analyte is bound;
- d) correlating the difference in the zeta potential with changes of zeta potential for known concentrations of the analyte and thereby determining the presence and amount of the analyte.
1 more element
Dependencies are inferred from the claim text.