Antibodies to LGE.sub.2 -protein antigens
AssigneeCase Western Reserve University
InventorRobert G. Salomon
Life of this patent
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Abstract
Levuglandin (LG) derivatives are used as antigens for raising antibodies useful in diagnostic assays. The antibodies produced by LG-carrier protein adducts can be used to detect adducts of LGE.sub.2 with human low density lipoprotein (LDL). LGE.sub.2 -protein adduct immunoreactivity may be generated during in vitro free-radical oxidation of LDL. An enzyme-linked immunosorbent assay for detecting adducts of LGE.sub.2 with human LDL is also described.
Claims20
A method of producing antibodies specific to levuglandin-carrier protein-bound pyrrole adducts comprising:
- a) reacting a levuglandin with a carrier protein to form a levuglandin-carrier protein-bound pyrrole adduct;
- b) injecting said levuglandin-carrier protein-bound pyrrole adduct into an animal under conditions such that antibodies specific to levuglandin-carrier protein-bound pyrrole adducts are produced;
- and
- c) collecting said antibodies from said animal.
A method of detecting levuglandin-carrier protein-bound pyrrole adducts in a patient having a disease associated with oxidative injury, comprising:
- a) providing:
- (i) antibodies to a levuglandin-carrier protein-bound pyrrole adduct, said adduct containing a levuglandin component and a protein component,
- (ii) a patient sample to be tested for the presence of antigens reactive with said antibodies;
- b) combining said sample and said antibodies to from a reaction solution;
- and
- c) screening said reaction solution for the presence of a reaction between said antigens and said antibodies.
Dependencies are inferred from the claim text.