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AssigneeForestry Canada
InventorAbul K. M. Ekramoddoullah
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A method of determining frost hardiness of a conifer seedling, which comprises detecting an amount of a cold protein in tissue of said conifer, the cold protein being a protein of approximately 19 kD that increase significantly in amount in the conifer during fall season (autumnal) months and that imparts frost hardiness to the seedling, and then assessing frost hardiness based on said detected amount. In the case of Pinus lambertiana, the cold protein includes the N-terminal sequence: Val--Ser--Gly--Thr--Ser--Ser--Thr--Glu--Glu--Val--Val--Gln--Asn--Glu--Ala- -Arg--Arg--Leu--Trp--Asn SEQ ID NO:1!; and in the case of Pinus monticola, the cold protein includes the N-terminal sequence: Val--Ser--Gly--Thr--Ser--Ser--Thr--Glu--Glu--Val--Val--Gln--Val--Glu--Ala- -Arg--Arg--Leu--Trp--Asn--Ala--Thr--Thr--Lys--Asp SEQ ID NO:3!. The invention also relates to conjugates and antibodies used in the method.
A method of determining frost hardiness of a conifer seedling, which comprises detecting an amount of a cold protein in tissue of said seedling, said cold protein being a protein of approximately 19 kD that increase significantly in amount in said conifer during fall season (autumnal) months and that is associated with seedlings having frost hardiness, and assessing frost hardiness based on said detected amount.
An antibody that binds to a cold protein of approximately 19 kD that increases significantly in amount in conifer seedlings during fall season (autumnal) months and that is associated with seedlings having frost hardiness.
A method of planting conifer in a region subject to freezing temperatures for improved resistance to damage by frost, comprising producing seedlings of said conifer, determining frost hardiness of said seedlings, eliminating seedlings determined not to be suitably frost hardy, and planting the remaining frost hardy seedlings in said region, wherein said frost hardiness of said seedlings is determined by detecting an amount of a cold protein in tissue of said seedling, said cold protein being a protein of approximately 19 kD that increase significantly in amount in said conifer during fall season (autumnal) months and that increase significantly in amount in said conifer during fall season months and that is associated with seedlings having frost hardiness, and assessing frost hardiness based on said detected amount.
A kit for determining frost hardiness of conifer seedlings which develop a cold protein in fall months that is associated with protection of said seedlings against frost, comprising a supply of an antibody that binds to said cold protein, a developing agent for indicating the presence of said antibody and equipment for enabling a sample of said tissue from said seedling to be exposed to said antibody and detected.
Dependencies are inferred from the claim text.