Back to results
US 5,686,244 AGranted
Method for detecting a nucleic acid analyte using an improved electrochemiluminescent label
AssigneeIgen, Inc.
InventorsSatyanarayana R. Gudibande, John H. Kenten
Life of this patent
Estimating the term…
Abstract
This invention relates to a new electrochemiluminescent (ECL) label for oligonucleotides using phosphoramidite chemistry.
Claims5
A method of detecting a nucleic acid analyte of interest present in a sample comprising the steps of contacting said sample with a probe of the formula ##STR23## wherein M is ruthenium, osmium or rhenium;
- R.sub.1, R.sub.2, R.sub.3, R.sub.4 and R.sub.5 are the same or different and each is H or alkyl of 1-4 carbon atoms;
- n is an integer from 1 to 20;
- X is selected from the group consisting of O, S, SO.sub.2, COO and CONH;
- y is an integer of from 1-20;
- z is 0 or 1;
- m is an integer of from 1-1000;
- and NAB is a nucleic acid base which may be modified or unmodified, under conditions wherein said probe selectively binds to said analyte of interest to form an analyte of interest--probe complex;
- and measuring the electrochemiluminescence of said complex.
5 more elements
A method for detecting a nucleic acid of interest in the product of a polymerase chain reaction of other primer-initiated, template-directed reaction comprising the steps of:
- (a) labeling at least one nucleic acid in said reaction with a compound of the formula ##STR24## wherein M is ruthenium, osmium or rhenium;
- R.sub.1, R.sub.2, R.sub.3, R.sub.4 and R.sub.5 are the same or different and each is H or alkyl of 1-4 carbon atoms;
- n is an integer from 1 to 20;
- X is selected from the group consisting of O, S, SO.sub.2, COO and CONH;
- y is an integer of from 1-20;
- z is 0 or 1;
- m is an integer of from 1-1000;
- and NAB is a nucleic acid base which may be modified or unmodified;
- (b) conducting a polymerase chain reaction or other primer-initiated, template-directed reaction which incorporates said nucleic acid into an amplification product;
- and
- (c) measuring the electrochemiluminescence of said nucleic acid of interest.
8 more elements
Dependencies are inferred from the claim text.