Method and device for testing blood units for viral contamination
AssigneeBaxter International Inc.
InventorsJeffrey Martinson, William Bratten, Li Ming Wang, John Chapman
Life of this patent
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Abstract
A method and apparatus for creating a leukocyte rich sample from a mixed population of blood cells. The method comprises the steps of: separating an amount of blood into layers that include a buffy coat layer having a mixed population of blood cells including plasma and red blood cells; providing a chamber having an opening for receiving a fluid; causing the buffy coat layer to enter the chamber; centrifuging the chamber to separate the buffy coat layer into at least a plasma containing layer, a leukocyte containing layer, and a red blood cell containing layer; and separating the leukocyte containing layer from the other layers in the chamber.
Claims28
A method for testing a blood unit collected from a donor for viral contamination without rendering at least a platelet rich plasma layer and a red blood cell rich layer of the blood unit unusable for therapeutic applications comprising the steps of:
- collecting the blood unit in a container;
- separating an amount of blood within the container using at least, in part, a centrifugation process, into a platelet rich plasma layer, a red blood cell rich layer, and a buffy layer that includes a mixed population of blood cells including plasma and red blood cells;
- providing a chamber having an opening in communication with the container for receiving a fluid;
- causing the buffy coat layer to enter the chamber while the platelet rich plasma layer and the red blood cell rich layer remain in the container;
- centrifuging the chamber to separate the buffy coat layer into at least a plasma containing layer, a leukocyte rich layer, and a red blood cell containing layer;
- separating the leukocyte rich layer from other layers in the chamber;
- and
- assaying the leukocyte rich layer to determine viral contamination.
A method for testing a blood unit for viral contamination without rendering at least either a platelet rich plasma layer or a red blood cell rich layer of the blood unit unusable comprising the steps of:
- collecting an amount of blood in a container;
- separating the amount of blood into layers that include a buffy coat layer that includes a mixed population of blood cells including plasma and red blood cells;
- providing a syringe having a cannula coupled to an opening for receiving a fluid, a chamber, and a plunger received within the chamber;
- piercing the container including a buffy coat layer with the cannula and causing the buffy coat layer to enter the chamber;
- securing means for preventing axial movement of the plunger into the chamber around at least a portion of the plunger;
- centrifuging the syringe to separate the buffy coat layer into at least a plasma containing layer, a leukocyte containing layer, and a red blood cell containing layer;
- and
- separating the leukocyte rich layer from the other layers in the chamber.
A method for testing a blood unit collected from a donor for viral contamination without rendering at least a platelet rich plasma layer and a red blood cell rich layer of the blood unit unusable for therapeutic applications comprising the steps of:
- separating, in a first container, an amount of blood using at least, in part, a centrifugation process, into a platelet rich plasma layer, a red blood cell rich layer, and a buffy coat layer that includes a mixed population of blood cells including plasma and red blood cells;
- providing a second container in communication with the first container for receiving the buffy coat layer;
- causing the buffy coat layer to enter the second container while the platelet rich plasma layer and the red blood cell rich layer remain in the first container;
- further centrifuging the second container to separate the buffy coat layer into at least a plasma containing layer, a leukocyte rich layer, and a red blood cell containing layer;
- separating the leukocyte rich layer from the plasma containing layer and red blood cell containing layer in the second container;
- and
- assaying the leukocyte rich layer to determine viral contamination.
Dependencies are inferred from the claim text.