# US 5,686,078 A · Primary and secondary immunization with different physio-chemical forms of antigen

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- Number: US 5,686,078 A
- Document ID: US5686078A
- Status: Granted
- Kind: A
- Application number: 08/385,586
- Filed: 1995-02-08
- Granted: 1997-11-11
- Earliest claimed: 1992-09-14
- Assignees: Connaught Laboratories Limited
- Inventors: Robert S. Becker; Laura Ferguson; Lorne Erdile; Maurice W. Harmon; Robert Huebner
- Web page: https://patentgrep.com/patent/US5686078A

## CPC

- A61K 39/145: Orthomyxoviridae, e.g. influenza virus
- A61K 39/0225: {Spirochetes, e.g. Treponema, Leptospira, Borrelia}
- A61K 39/12: Viral antigens
- A61K 2039/5252: {inactivated (killed)}
- A61K 2039/545: {characterised by the dose, timing or administration schedule}
- A61K 2039/70: {Multivalent vaccine}
- C12N 2760/16134: Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
- C12N 2760/16234: Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
- Y02A 50/30: Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE

## Abstract

Animals, including humans, are immunized by antigens, for example, the HA antigen of influenza, by first administering to a naive animal a normally strongly-immunogenic form of the antigen, for example, inactiviated or attenuated whole cell virus and subsequently administering a normally weakly-immunogenic isolated and purified viral antigen, to achieve an enhanced immune response to the purified viral antigen.

## Claims

### Claim 1

A method of achieving an immune reaction to a highly-immunogenic form of a viral bacterial antigen in an animal, which consists essentially of the steps in the sequence set forth:
administering by vaccination to said animal the highly-immunogenic form of said antigen to achieve a primary immune reaction to the antigen, wherein the highly-immunogenic form of said antigen is particulate, and
subsequently administering by vaccination to said animal a form of said antigen which is weakly-immunogenic and capable of recalling the memory immune reaction to the highly-immunogenic form of the antigen, to achieve a booster immune reaction to the antigen, wherein said weakly-immunogenic form of said antigen is soluble.

### Claim 2 (depends on 1)

The method of claim 1 wherein said highly immunogenic form of the antigen is inactivated or attenuated whole cell virus.

### Claim 3 (depends on 2)

The method of claim 2 wherein said weakly-immunogenic form of the antigen is an isolated and purified antigen.

### Claim 4 (depends on 3)

The method of claim 3 wherein said antigen is isolated and purified from whole cells.

### Claim 5 (depends on 3)

The method of claim 3 wherein said antigen is produced synthetically.

### Claim 6 (depends on 3)

The method of claim 3 where said antigen is the HA(p) antigen from influenza virus.

### Claim 7 (depends on 3)

The method of claim 3 wherein said antigen is the gp120 protein from HIV.

### Claim 8 (depends on 1)

A method of claim 1 wherein the antigen is a bacterial antigen.

### Claim 9 (depends on 8)

The method of claim 8 wherein said antigen is OspA-NL from B. burgdorferi.

### Claim 10 (depends on 1)

The method of claim 1 wherein said animal is a human.