Synthetic peptide vaccines for dental caries
AssigneeForsyth Dental Infirmary for Children
InventorsMartin A. Taubman, Daniel J. Smith
Life of this patent
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Abstract
Immunization of animals with a composition containing either an amino acid sequence from the catalytic domain of glucosyltransferase, an amino acid sequence from the glucan-binding region of glucosyltransferase or an amino acid sequence from the native surface domain of glucosyltransferase provoke antibody and T-cell immune responses to this enzyme. Since this enzyme has been implicated in the colonization of mutans streptococci on tooth surfaces, such immune responses are important for the prevention of dental caries. Multicomponent and multivalent compositions which include these amino acid sequences provide effective vaccine capabilities.
Claims21
An immunogenic composition comprising a peptide consisting of at least one amino acid sequence selected from the group consisting of:
- a) DGKLRYYDANSGDQAFNKSV (SEQ ID NO:
- 4), and
- b) PLDKRSGLNPLIHNSLVDREVDDRE (SEQ ID NO:
- 2);
- and a physiologically compatible carrier.
An immunogenic composition comprising at least two peptides, wherein at feast one peptide consists of an amino acid sequence of either DANFDSIRVDAVDNVDADLLQI (SEQ ID NO:
- 1) or PLDKRSGLNPLIHNSLVDREVDDRE (SEQ ID NO:
- 2) where both sequences are of the catalytic domain of streptococcal glucosyltransferase, and at least one peptide consists of an amino acid sequence of either TGAQTIKGQKLYFKANGQQVKG (SEQ ID NO:
- 3) or DGKLRYYDANSGDQAFNKSV (SEQ ID NO:
- 4) where both sequences are of the glucan-binding domain of streptococcal glucosyltransferase, and a physiologically compatible carrier.
An immunogenic composition comprising at least two peptides of a streptococcal glucosyltransferase protein covalently attached to a lysine core matrix, wherein each peptide consists of an amino acid sequence selected from the group consisting of:
- a) DANFDSIRVDAVDNVDADLLQI (SEQ ID NO:
- 1),
- b) TGAQTIKGQKLYFKANGQQVKG (SEQ ID NO:
- 3),
- c) DGKLRYYDANSGDQAFNKSV (SEQ ID NO:
- 4),
- d) QWNGESEKPYDDHL (SEQ ID NO:
- 5), and
- e) PLDKRSGLNPLIHNSLVDREVDDRE (SEQ ID NO:
- 2);
- and
- a physiologically compatible carrier.
An immunogenic composition comprising a peptide consisting of an amino acid sequence of PLDKRSGLNPLIHNSLVDREVDDRE (SEQ ID NO:
- 2) and a physiologically compatible carrier.
A method of interfering with the enzymatic activity of streptococcal glucosyltransferase in a mammal comprising the administration of a peptide consisting of an amino acid sequence of PLDKRSGLNPLIHNSLVDREVDDRE (SEQ ID NO:
- 2) to a mammal in a manner that raises an immune response in the mammal, thereby interfering with the enzymatic activity of streptococcal glucosyltransferase in the mammal.
A method of provoking an immune response to streptococcal glucosyltransferase in a mammal comprising the administration of a peptide consisting of an amino acid sequence of either PLDKRSGLNPLIHNSLVDREVDDRE (SEQ ID NO:
- 2) or DGKLRYYDANSGDQAFNKSV (SEQ ID NO:
- 4) in a manner that raises an immune response in the mammal.
An immunogenic composition comprising a peptide consisting of an amino acid sequence of DGKLRYYDANSGDQAFNKSV (SEQ ID NO:
- 4) and a physiologically compatible carrier.
A method of interfering with the glucan-binding activity of streptococcal glucosyltransferase in a mammal comprising the administration of a peptide consisting of an amino acid sequence of DGKLRYYDANSGDQAFNKSV (SEQ ID NO:
- 4) in a manner that induces a response which thereby interferes with the glucan-binding activity of streptococcal glucosyltransferase in the mammal.
An immunogenic composition comprising at least two peptides covalently attached to at least one additional immunologic component which produces an immunogenic response against an infectious organism, wherein each peptide is selected from the group consisting of:
- a) DANFDSIRVDAVDNVDADLLQI (SEQ ID NO:
- 1);
- b) PLDKRSGLNPLIHNSLVDREVDDRE (SEQ 1D NO:
- 2);
- c) TGAQTIKGQKLYFKANGQQVKG (SEQ ID NO:
- 3);
- d) DGKLRYYDANSGDQAFNKSV (SEQ ID NO:
- 4);
- and
- e) QWNGESEKPYDDHL (SEQ ID NO:
- 5);
- and
- a physiologically compatible carrier, wherein said additional immunologic component is a peptide comprising an amino acid sequence from an immunologic domain selected from the group consisting of diphtheria, pertussis, tetanus and measles.
An immunogenic composition comprising a peptide covalently attached to at least one additional immunologic component which produces an immunogenic response against an infectious organism, wherein said peptide is selected from the group consisting of:
- a) DANFDSIRVDAVDNVDADLLQI (SEQ ID NO:
- 1);
- b) PLDKRSGLNPLIHNSLVDREVDDRE (SEQ ID NO:
- 2);
- c) TGAQTIKGQKLYFKANGQQVKG (SEQ ID NO:
- 3);
- d) DGKLRYYDANSGDQAFNKSV (SEQ ID NO:
- 4);
- and
- e) QWNGESEKPYDDHL (SEQ ID NO:
- 5);
- and
- a physiologically compatible carrier, wherein said additional immunologic component is a peptide comprising an amino acid sequence from an immunologic domain selected from the group consisting of diphtheria, pertussis, tetanus and measles.
Dependencies are inferred from the claim text.