Polyclonal and monoclonal antibodies against a 43 KDA dystrophin associated protein
AssigneeUniversity of Iowa Research Foundation
InventorsKevin P. Campbell, Oxana B. Ibraghimov, James M. Ervasti, Cynthia J. Leveille
Life of this patent
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Abstract
Disclosed are methods for the preparation of polyclonal and monoclonal antibodies which bind specifically to a 43 kDa dystrophin-associated. The molecular weight of the 43 kDa protein is determined by electrophoretic separation under denaturing conditions, followed by transfer to a solid support and staining with wheat germ agglutinin. The method includes a step in which the peptide PKNMTPYRSPPPYVP (SEQ ID NO: 15) is administered to stimulate an immune response. Also disclosed are polyclonal and monoclonal antibodies which bind specifically to the 43 kDa dystrophin-associated protein.
Claims6
A method for producing a polyclonal antibody which binds specifically to the 43 kDa dystrophin-associated protein, the molecular weight of the protein being determined by electrophoretic separation under denaturing conditions, transfer to a solid support and staining with wheat germ agglutinin, the method comprising:
- a) providing the peptide PKNMTPYRSPPPYVP (SEQ ID NO:
- 15);
- b) administering the peptide to a mammal under conditions appropriate for the stimulation of an immune response;
- and
- c) isolating the polyclonal antibody from the mammal.
A method for producing a monoclonal antibody which binds specifically to the 43 kDa dystrophin-associated protein, the molecular weight of the protein being determined by electrophoretic separation under denaturing conditions, transfer to a solid support and staining with wheat germ agglutinin, the method comprising:
- a) providing the peptide PKNMTPYRSPPPYVP (SEQ ID NO:
- 15);
- b) administering the peptide to a mammal under conditions appropriate for the stimulation of an immune response;
- c) isolating antibody producing cells from the mammal;
- d) fusing the antibody producing cells with immortalizing cells to produce a hybridoma cell line;
- and
- e) screening the resulting hybridoma cell line to identify cell lines secreting antibody having the desired specificity.
An immunogenic peptide PKNMTPYRSPPPYVP (SEQ ID NO:
- 15) which, in the immunization of a mammal, stimulates the production of antibodies which bind specifically to the 43 kDa dystrophin-associated protein, the molecular weight of the protein being determined by electrophoretic separation under denaturing conditions, transfer to a solid support and staining with wheat germ agglutinin.
Dependencies are inferred from the claim text.