Purified tobacco protein involved in nicotine synthesis, DNA encoding, and use of sense and antisense DNAs corresponding thereto to affect nicotine content in tobacco plants
AssigneePhilip Morris Incorporated
InventorsHerbert Y. Nakatani, Vedpal S. Malik
Life of this patent
Estimating the term…
Abstract
Nicotine acid sequences encoding a tobacco protein involved in nicotine synthesis are described. These sequences, when inserted in to sense or anti-sense orientation, affect nicotine synthesis in transgenic tobacco plants.
Claims8
An isolated DNA molecule encoding an RNA that hybridizes to an mRNA encoding a protein having an amino acid sequence defined by SEQ ID NO:1, SEQ ID NO:2 or SEQ ID NO:3 and which DNA molecule when stably transformed into a tobacco plant is capable of lowering the nicotine content therein in comparison to an untransformed control tobacco plant by affecting the expression of a protein containing a sequence defined by SEQ ID NO:1, SEQ ID NO:2 or SEQ ID NO:3.
An isolated DNA molecule encoding an mRNA which encodes for a polypeptide comprising at least a portion of a protein having an amino acid defined by SEQ ID NO:1, SEQ ID NO:2 or SEQ ID NO:3 wherein said isolated DNA when stably transformed into a tobacco plant is capable of lowering the nicotine content therein in comparison to an untransformed control tobacco plant by affecting the expression of a protein containing a sequence defined by SEQ ID NO:1, SEQ ID NO:2 or SEQ ID NO:3.
Dependencies are inferred from the claim text.