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AssigneeAmoco Corporation
InventorsRodney L. Ausich, Friedhelm Luetke Brinkhaus, Indrani Mukharji, John H. Proffitt, James G. Yarger, Huei-Che Bill Yen
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DNA segments encoding the Erwinia herbicola enzymes geranylgeranyl pyrophosphate (GGPP) synthase, phytoene synthase, phytoene dehydrogenase-4H, lycopene cyclase, beta-carotene hydroxylase, and zeaxanthin glycosylase, DNA variants and analogs thereof encoding an enzyme exhibiting substantially the same biological activity, vectors containing those DNA segments, host cells containing the vectors and methods for producing those enzymes, zeaxanthin and zeaxanthin diglucoside by recombinant DNA technology in transformed host organisms are disclosed.
A purified and isolated DNA segment comprising a nucleotide sequence that contains at least 531 base pairs that define the structural gene for the Erwinia herbicola enzyme beta-carotene hydroxylase that is present in a restriction endonuclease fragment comprising the approximately 870 base pair Nco I-Sma I restriction fragment of plasmid pARC406BH, the approximately 1797 base pair Hind III-Hind III fragment of plasmid pARC429BH or the approximately 900 base pair Nco I-Hind III fragment of plasmid pARC145H, said beta-carotene hydroxylase converting beta-carotene into zeaxanthin.
A purified and isolated DNA segment comprising a nucleotide sequence that contains at least 531 base pairs that define a structural gene for the Erwinia herbicola enzyme beta-carotene hydroxylase that is present in a plasmid selected from the group consisting of pARC406BH, pARC429BH and pARC145H or a DNA variant that has at least 80 percent identity to said gene and hybridizes with said gene under moderately high stringency conditions comprising hybridization at a temperature of about 50.degree.-55.degree. C. in 6.times.SSC and a final wash at a temperature of 68.degree. C. in 1-3.times.SSC, said nucleotide sequence encoding an enzyme that converts beta-carotene into zeaxanthin.
A recombinant DNA molecule comprising a vector operatively linked to an exogenous DNA segment that contains at least 531 base pairs defining a structural gene for the Erwinia herbicola enzyme beta-carotene hydroxylase that is present in a plasmid selected from the group consisting of pARC406BH, pARC429BH and pARC145H or a DNA variant that has at least 80 percent identity to said gene and hybridizes with said gene under moderately high stringency conditions comprising hybridization at a temperature of about 50.degree.-55.degree. C. in 6.times.SSC and a final wash at a temperature of 68.degree. C. in 1-3.times.SSC, said nucleotide sequence encoding an enzyme that converts beta-carotene into zeaxanthin, and a promoter for driving the expression of said enzyme in a host organism.
A transformed higher plant whose genome contains
A purified and isolated DNA segment comprising a nucleotide sequence that contains at least 1200 base pairs that define the structural gene for the Erwinia herbicola enzyme zeaxanthin glycosylase present in a restriction endonuclease fragment that is the approximately 1390 base pair Nde I-Ava I restriction fragment of plasmid pARC2019, said zeaxanthin glycosylase converting zeaxanthin into zeaxanthin diglucoside.
A purified and isolated DNA segment comprising a nucleotide sequence that contains at least 1200 base pairs that define a structural gene for the Erwinia herbicola enzyme zeaxanthin glycosylase present in plasmid pARC2019 or a DNA variant that has at least 80 percent identity to said gene and hybridizes with said gene under moderately high stringency conditions comprising hybridization at a temperature of about 50.degree.-55.degree. C. in 6.times.SSC and a final wash at a temperature of 68.degree. C. in 1-3.times.SSC, said nucleotide sequence encoding an enzyme that converts zeaxanthin into zeaxanthin diglucoside.
A recombinant DNA molecule comprising a vector operatively linked to an exogenous DNA segment that contains at least 1200 base pairs defining a structural gene for the Erwinia herbicola enzyme zeaxanthin glycosylase that is present in plasmid pARC2019 or a DNA variant that has at least 80 percent identity to said gene and hybridizes with said gene under moderately high stringency conditions comprising hybridization at a temperature of about 50.degree.-55.degree. C. in 6.times.SSC and a final wash at a temperature of 68.degree. C. in 1-3.times.SSC, said nucleotide sequence encoding an enzyme that converts zeaxanthin into zeaxanthin diglucoside, and a promoter for driving the expression of said enzyme in a host organism.
A transformed higher plant whose genome contains
Plasmid pARC406BH having ATCC accession number 40945.
Plasmid pARC145H having ATCC accession number 40944.
Plasmid pARC404BH having ATCC accession number 40943.
Plasmid pARC2019 having ATCC accession number 40974.
Dependencies are inferred from the claim text.