# US 5,684,145 A · Carrier system against GNRH

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- Number: US 5,684,145 A
- Document ID: US5684145A
- Status: Granted
- Kind: A
- Application number: 08/453,588
- Filed: 1995-05-30
- Granted: 1997-11-04
- Earliest claimed: 1992-06-18
- Assignees: Akzo Nobel N.V.
- Inventors: Anna Van Der Zee; Irma Marianne Van Die; Willem Pieter Martin Hoekstra; Josephus Theodorus Gielen
- Web page: https://patentgrep.com/patent/US5684145A

## CPC

- C07K 7/23: Luteinising hormone-releasing hormone [LHRH]; Related peptides
- A61P 13/02: of urine or of the urinary tract, e.g. urine acidifiers
- A61P 15/00: Drugs for genital or sexual disorders (for disorders of sex hormones A61P5/24); Contraceptives
- A61P 37/04: Immunostimulants
- C07K 14/245: Escherichia (G)
- A61K 39/00: Medicinal preparations containing antigens or antibodies (materials for immunoassay G01N33/53)
- C07K 2319/00: Fusion polypeptide
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)

## Abstract

The present invention is concerned with vaccination of mammals against GnRH. The vaccine comprises a GnRH peptide conjugate to E. coli fimbrial-filaments and elicits an immune response against GnRH.

## Claims

### Claim 1

A recombinant DNA molecule that codes for a hybrid protein, said hybrid protein comprising: an E. coli P-fimbrial filament that contains a major subunit thereof; and an antigenic determinant of GnRH or an analog or derivative of GnRH, which is inserted in hypervariable region 4 (HR4) of the major subunit, wherein said major subunit further comprises a mutation consisting of the creation of a StuI site in hypervariable region 1.

### Claim 2 (depends on 1)

A microorganism comprising a recombinant DNA molecule according to claim 1, wherein said microorganism expresses said hybrid protein.

### Claim 3 (depends on 2)

A microorganism according to claim 2, which allows for biogenesis of fimbriae.

### Claim 4 (depends on 3)

A vaccine capable of eliciting an immune response against GnRH in an animal, said vaccine comprising an effective amount of a microorganism according to claim 3.

### Claim 5 (depends on 1)

An expression vector comprising a recombinant DNA molecule according to claim 1.

### Claim 6 (depends on 5)

A microorganism comprising an expression vector according to claim 5, wherein said microorganism expresses said hybrid protein.

### Claim 7 (depends on 6)

A microorganism according to claim 6, which allows for biogenesis of fimbriae.

### Claim 8 (depends on 7)

A vaccine capable of eliciting an immune response against GnRH in an animal said vaccine comprising an effective amount of a microorganism according to claim 7.