Antibody specific for .beta.1.fwdarw.6 N-acetylglucosamininyltransferase
AssigneeLa Jolla Cancer Research Center
InventorsMinoru Fukuda, Marti F. A. Bierhuizen
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Abstract
The present invention provides a novel .beta.1.fwdarw.6 N-acetylglucosaminyltransferase, which forms core 2 oligosaccharide structures in O-glycans, and a novel acceptor molecule, leukosialin, CD43, for core 2 .beta.1.fwdarw.6 N-acetylglucosaminyltransferase activity. The amino acid sequences and nucleic acid sequences encoding these molecules, as well as active fragments thereof, also are disclosed. A method for isolating nucleic acid sequences encoding proteins having enzymatic activity is disclosed, using CHO cells that support replication of plasmid vectors having a polyoma virus origin of replication. A method to obtain a suitable cell line that expresses an acceptor molecule also is disclosed.
Claims4
An isolated antibody or an antigen binding antibody fragment having specific reactivity for human core 2 .beta.1.fwdarw.6 N-acetylglucosaminyltransferase having activity for a core 1-containing acceptor molecule.
Dependencies are inferred from the claim text.