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US 5,683,909 AGranted
Plasmids replicatable in Bacillus subtilis, E. coli and lactic acid streptococcus bacteria
AssigneeVan den Bergh Foods Co., Division of Conopco, Inc.
InventorsJan Kok, Jan Maat, Josephus Mauritius van der Vossen, Gerard Venema
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Abstract
The claimed invention is drawn to a recombinant plasmid which can replicate in Bacillus subtilis, Escherichia coli, and lactic acid Streptococcus bacteria comprising the replication of origin from Streptococcus cremoris plasmid pWV01 as its origin of replication, in addition to coding marker genes and genes of interest which code for improved fermenting properties.
Claims10
A recombinant plasmid capable of replication in each of the microorganisms Bacillus subtilis, Escherichia coli and lactic acid streptococcus bacteria, comprising
- (i) at least the part of the streptococcus cremoris plasmid pWV01 which carries the replication origin for expression in streptococcus cremoris,
- (ii) at least one coding marker capable of expression in said microorganisms, and
- (iii) at least one piece of insert-DNA expressible in said microorganisms to give said microorganism improved fermenting properties.
Dependencies are inferred from the claim text.