Metal ion-ligand coordination complexes, antibodies directed thereto, and assays using such antibodies
AssigneeAbbott Laboratories
InventorDavid K. Johnson
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Abstract
The present invention relates to the field of immunossays for metal ions. The invention presents: metal ion-ligand coordination complexes ("MLC"), novel ligands, antibodies specific for MLC, immunoassays utilizing the foregoing, and methods for selecting said antibodies.
Claims2
An affinity chromatography medium wherein a metal ion-ligand coordination complex consisting of two or more ligands, wherein said ligands are bifunctional, is covalently bonded to a solid support, said ligands being selected from the group consisting of:
- ##STR1##
An assay method for screening and characterizing monoclonal antibodies that are reactive with a metal ion-ligand coordination complex, comprising the steps of:
- (a) covalently coupling a pre-formed metal ion-ligand coordination complex to a protein, to form a metal ion-ligand coordination complex-protein conjugate;
- (b) incubating the solid phase from step (a) with a sample thought to contain monoclonal antibodies reactive with the metal ion-ligand coordination complex
- (c) washing the solid phase and detecting bound antibody by exposing the solid phase to anti-mouse antibody that is labeled with a signal-generating moiety;
- and
- (d) comparing the results from step (c) with those of analogous assays in which, at step (a), the metal ion of interest is replaced by a potentially crossreactive metal ion, said ligand of said metal ion-ligand coordination complex selected from the group consisting of:
- ##STR2##
Dependencies are inferred from the claim text.