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US 5,683,905 AGranted
Vectors and cell lines capable of correctly splicing exon regions
AssigneeGenentech, Inc.
InventorsDaniel J. Capon, Richard M. Lawn, Gordon A. Vehar, William I. Wood
Life of this patent
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Abstract
A recombinant DNA vector is provided that expresses exons of genomic DNA fragments that are inserted into the vector. The vector contains a promoter and a genomic DNA fragment so characterized and configured that the vector, upon transcription in a transfected eukaryotic cell culture, expresses the corresponding RNA segment of the genomic DNA fragment free of any intron.
Claims6
A recombinant DNA vector which expresses exons of genomic DNA fragments inserted into the vector, which vector comprises:
- (1) a promoter operably linked to a splice donor site followed by a splice acceptor site and (2) a genomic DNA fragment inserted between said splice donor and splice acceptor sites, wherein the genomic DNA fragment comprises a splice acceptor site followed by a splice donor site, and wherein said vector, upon transcription in a transfected eukaryotic cell culture, expresses the corresponding RNA segment of said genomic DNA fragment free of any intron.
pESVDA.
Dependencies are inferred from the claim text.