US 5,683,900 AGranted

Pasteurella haemolytica PhaI restriction endonuclcape and methyltranstesase

AssigneesThe United States of America as represented by the Department of Agriculture, Biotechnology Research and Development Corporation

InventorsRobert E. Briggs, Fred M. Tatum

Life of this patent

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Abstract

Methylation of DNA can be a critical step in the introduction of DNA into P. haemolytica. A methyltransferase has been isolated and molecularly cloned for this purpose. Use of the methyltransferase has allowed construction of defined, attenuated mutants for use as vaccines to protect cattle.

Claims4

Dependent claims

A preparation of PhaI methyltransferase free from PhaI restriction endonuclease.

Dependencies are inferred from the claim text.

Facts

Application: 08/643,300Filed 8 May 1996 · division
Priority: 6 Dec 1993
Granted: 4 Nov 1997
Term ends: Not estimatedThe source records don’t support an estimate.

Classification10 codes

C12N 9/1092
Transferring alkyl or aryl groups other than methyl groups: 3-Phosphoshikimate 1-carboxyvinyltransferase , i.e. 5-enolpyruvylshikimate-3-phosphate…
A61K 39/102
Bacterial antigens: pasteurellales, e.g. Actinobacillus, Pasteurella; Haemophilus
C07K 14/285
From bacteria: from Pasteurellaceae , e.g. Haemophilus influenza
C12N 9/1007
Transferring one-carbon groups: methyltransferases

Family

Serial162,392Parent of this division · filed 6 Dec 1993
US 5,587,305 AParent of this division
US 5,683,900 AThis patent

From the US filing history. Foreign family members aren’t listed.

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