Enzymatic process for the production of dihydroxyacetone phosphate from glycerophosphate and its use in enzymatic aldol additions
AssigneeBoehringer Mannheim GmbH
InventorWolf-Dieter Fessner
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Abstract
The invention concerns a process for the production of dihydroxyacetone phosphate (DHAP) by enzymatic oxidation of glycerophosphate in the presence of glycerophosphate oxidase and an H.sub.2 O.sub.2 -decomposing enzyme such as catalase and also concerns the conversion of DHAP formed in situ in a coupled enzymatic aldol addition to produce carbohydrates or corresponding derivatives.
Claims15
A method for producing dihydroxyacetone phosphate comprising oxidizing at least one member selected from the group consisting of L-glycerol monophosphate and a racemic mixture of DL-glycerol monophosphate with glycerophosphate oxidase in the presence of catalase and a member selected from the group consisting of oxygen, air, and hydrogen peroxide.
A method for producing a sugar or sugar derivative from glycerol monophosphate comprising oxidizing glycerol monophosphate with glycerophosphate oxidase in the presence of catalase and a member selected from the group consisting of oxygen, air and hydrogen peroxide, wherein said oxidizing is performed in the presence of an aldolase and an aldehyde.
A method for producing dihydroxyacetone phosphate comprising oxidizing L-glycerol monophosphate with glycerophosphate oxidize in the presence of catalase and a member selected from the group consisting of oxygen, air, and hydrogen peroxide.
A method for producing dihydroxyacetone phosphate comprising oxidizing a racemic mixture of DL-glycerol monophosphate with glycerophosphate oxidase, in the presence of catalase and a member selected from the group consisting of oxygen, air, and hydrogen peroxide.
A method for producing a sugar or sugar derivative from at least one member selected from the group consisting of L-glycerol monophosphate and a racemic mixture of DL-glycerol monophosphate comprising oxidizing said at least one of said L-glycerol monophosphate and said racemic mixture of DL-glycerol monophosphate with glycerophosphate oxidase in the presence of catalase and a member selected from the group consisting of oxygen, air and hydrogen peroxide, wherein said oxidizing is performed in the presence of an aldolase and an aldehyde.
Dependencies are inferred from the claim text.