9-deazaadenosine and its 5'-.alpha.-D-glycopyranoside isolated from the cyanobacterium Anabaena affinis strain VS-1
OwnerKenneth L. Rinehart, Wayne W. Carmichael +1inventors
InventorsKenneth L. Rinehart, Wayne W. Carmichael, Michio Namikoshi
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Abstract
The present invention is based upon the discovery that a MeOH extract of Anabaena affinis strain VS-1 showed strong cytotoxicity to L1210 murine leukemia cells, and from that extract was isolated the compounds having the following structures, Compounds 1 and 2. These two compounds are believed to be responsible for the cytotoxicity of the marine organism. This is the first report of the isolation and characterization of pyrrolo3,2-d!pyrimidine derivatives as biosynthetic products. ##STR1##
Claims10
A process for the biosynthetic production of the compound 9-deazaadenosine, comprising the steps of:
- (a) cultivating the cyanobacterium Anabaena affinis strain VS-1, ATCC No. 55755, in a nutrient medium until an isolatable quantity of 9-deazaadenosine is produced therein:
- (b) collecting and lyophilizing the cultured cells;
- (c) extracting the lyophilized cells with aqueous alcohol;
- and
- (d) purifying the alcohol extract by chromatographic means, thereby affording the purified compound 9-deazaadenosine.
A process for the biosynthetic production of the 5'-.alpha.-D-glycopyranoside of 9-deazaadenosine, comprising the steps of:
- (a) cultivating the cyanobacterium Anabaena affinis strain VS-1, ATCC No. 55755, in a nutrient medium until an isolatable quantity of the 5'-.alpha.-D-glycopyranoside of 9-deazaadenosine is produced therein:
- (b) collecting and lyophilizing the cultured cells;
- (c) extracting the lyophilized cells with aqueous alcohol;
- and
- (d) purifying the alcohol extract by chromato graphic means, thereby affording purified 5'-.alpha.-D-glycopyranoside of 9-deazaadenosine.
Dependencies are inferred from the claim text.