Method for producing exogenous protein
AssigneeJuridical Foundation The Chemo-Sero-Therapeutic Research Institute
InventorsKiyoto Nishiyama, Yuji Ishikawa, Kazuhiko Kimachi, Hiroaki Maeda, Sachio Tokiyoshi
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Abstract
A novel method for production of an exogenous protein is provided, the method being suitable for expression of an exogenous protein, especially a recombinant antibody etc., in an eucaryotic cell by utilizing a genetic recombination technique. That is, a novel method for production of an exogenous protein is provided which allows for culture of a cell in which an exogenous gene is introduced in a serum-free medium and an efficient production of an exogenous protein, by using, as a host cell for expression, a fused cell which is prepared by fusing a mouse myeloma and a lymphatic cell and which can be cultured in a serum-free medium.
Claims10
A method of producing an exogenous protein, comprising the steps of:
- obtaining a fused cell as a host cell for producing the exogenous protein, said fused cell prepared by fusing a mouse myeloma cell and a cell selected from the group consisting of a lymphatic cell and a lymphatic hybrid cell and isolating a fused cell which does not secrete an endogenous antibody of said lymphatic cell or of said lymphatic hybrid cell;
- incorporating an exogenous gene encoding for the exogenous protein into said mouse myeloma cell prior to said step of obtaining a fused cell or into said fused cell after said step of obtaining a fused cell;
- culturing said fused cell having said incorporated exogenous gene in a serum-free medium to express the exogenous protein;
- and
- collecting the exogenous protein from said medium.
Dependencies are inferred from the claim text.