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US 5,683,881 AGranted
Method of identifying sequence in a nucleic acid target using interactive sequencing by hybridization
AssigneeBiota Corp.
InventorSteven S. Skiena
Life of this patent
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Abstract
A new approach is proposed for sequencing by hybridization (SBH), which uses interaction to dramatically reduce the number of oligonucleotides used for de novo sequencing of large DNA fragments, while preserving the parallelism which is the primary advantage of SBH. In particular, a series of rounds is performed, starting from an initial fixed oligonucleotide array, of hybridizing a target sample against an array, and then designing a new oligonucleotide array in response to the results of the rounds to date, until the sequence is determined.
Claims14
A method of identifying a sequence of a nucleic acid target, said method comprising:
- (a) providing a first set of nucleic acid probes;
- (b) hybridizing said target with said first set;
- (c) identifying any probe(s) of said first set which is(are) positively and negatively hybridized with said target, said any positively hybridized probe(s) forming a first subset;
- (d) utilizing said first set and said first subset so as to select a second set of probes wherein said second set of probes contains within the nucleic acid sequence of the second set of probes at least a portion of the nucleic acid sequence of the positively hybridized probes of the first set;
- (e) providing said second set of probes;
- (g) identifying any probe(s) of said second set which is(are) positively and negatively hybridized with said target, said any positively hybridized probe(s) of said second set forming a second subset;
- and
- (h) utilizing said first and second sets and said first and second subsets so as to determine the sequence of said target.
5 more elements
Dependencies are inferred from the claim text.