Method for identifying a nucleotide base pair at a point mutation site in a DNA target using a mismatch repair enzyme

A method for identifying a nucleotide base pair at a point mutation site in a target double-stranded DNA molecule for which the corresponding wild-type DNA sequence has been determined, using a endonuclease that recognizes a base pair mismatch consisting of a first base and a second base, comprising:

• (A) denaturing said target double-stranded DNA molecule comprising said point mutation site into a first single-stranded target DNA molecule and a second eingle-stranded target DNA molecule, wherein said first single-stranded target DNA molecule comprises a first DNA sequence and said second single-stranded target DNA molecule comprises a second DNA sequence complementary to said first DNA sequence;
• (B) preparing a first, a second, a third and a fourth DNA probe, each comprising said mutation site, wherein said first and said second probes comprise said first DNA sequence, and said third and said fourth probes comprise said second DNA sequence, and wherein said first and said third probes contain at the mutation site said first base recognized by said mismatch endonuclease, and said second and said fourth probes contain at the mutation site said second base recognized by said mismatch endonuclease;
• (C) hybridizing said first, second, third and fourth DNA probes with the resulting single-stranded target DNA molecules of step (A) to form DNA probe-target DNA hybrid molecules comprising said mutation site in individual reaction vessels in the following manner:
• (1) said first DNA probe labelled with a detectable marker is hybridized to said second single-stranded target DNA molecule;
• (2) said second DNA probe is hybridized to said second single-stranded target DNA molecule labelled with a detectable marker;
• (3) said third DNA probe labelled with a detectable marker is hybridized to said first single-stranded target DNA molecule;
• and
• (4) said fourth DNA probe is hybridized to said first single-stranded target DNA molecule labelled with a detectable marker;
• (D) exposing the resulting hybrid molecules of step (C) to said endonuclease, wherein said endonucleaee recognizes a mismatch base pair selected from the group consisting of A/G, A/C, T/G and T/C, and
• (1) binds to said mismatched base pair in said hybrid molecules to form an enzyme-DNA complex which is labelled, or
• (2) cleaves a errand containing said mismatched base to produce a cleaved DNA fragment which is labelled;
• and
• (E) assaying for the presence of said enzyme-DNA complex which is labelled or said cleaved DNA fragment which is labelled,
• (1) wherein when said endonuclease recognizes A/G or A/C, and forms a labelled enzyme-DNA complex or produces labelled cleaved DNA fragments when exposed to the hybrid molecule obtained in step (C) (4), said nucleotide base pair at said point mutation site is A/T,
• (2) wherein when said endonuclease recognizes T/G or T/C, and forms a labelled enzyme-DNA complex or produces labelled cleaved DNA fragments when exposed to the hybrid molecule obtained in step (C) (2), said nucleotide base pair at said point mutation site is A/T,
• (3) wherein when said endonuclease recognizes A/G or A/C, and forms a labelled enzyme-DNA complex or produces labelled cleaved DNA fragments when exposed to the hybrid molecule obtained in step (C) (2), said nucleotide base pair at said point mutation site is T/A,
• (4) wherein when said endonuclease recognizes T/G or T/C, and forms a labelled enzyme-DNA complex or produces labelled cleaved DNA fragments when exposed to the hybrid molecule obtained in step (C) (4), said nucleotide base pair at said point mutation site is T/A,
• (5) wherein when said endonucleaee recognizes A/G or T/G, and forms a labelled enzyme-DNA complex or produces labelled cleaved DNA fragments when exposed to the hybrid molecule obtained in step (C) (3), said nucleotide base pair at said point mutation site is G/C,
• (6) wherein when said endonuclease recognizes A/C or T/C, and forms a labelled enzyme-DNA complex or produces labelled cleaved DNA fragments when exposed to the hybrid molecule obtained in step (C) (1), said nucleotide base pair at said point mutation site te G/C,
• (7) wherein when said endonuclease recognizes A/G or T/G, and forms a labelled enzyme-DNA complex or produces labelled cleaved DNA fragments when exposed to the hybrid molecule obtained in step (C) (1), said nucleotide base pair at said point mutation site is C/G, and
• (8) wherein when said endonuclease recognizes A/C or T/C, and forms a labelled enzyme-DNA complex or produces labelled cleaved DNA fragments when exposed to the hybrid molecule obtained in step (C) (3), said nucleotide base pair at said point mutation site is C/G.