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AssigneeResearch Corporation Technologies, Inc.
InventorEric T. Kool
Estimating the term…
The present invention provides single-stranded circular oligonucleotides each with at least one parallel binding (P) domain and/or at least one corresponding anti-parallel binding (AP) domain separated from each other by loop domains. When more than one P or AP domain is included in a circular oligonucleotide of the present invention, the additional P or AP domains can constitute loop domains for a pair of corresponding P and AP domains, and vice versa. The present invention further provides single-stranded circular oligonucleotides with at least one Hoogsten antiparallel (HAP) domain. Each P, AP and HAP domain has sufficient complementarity to bind to one strand of a defined nucleic acid target wherein the P domain binds in a parallel manner to the target and the HAP or AP domain binds in an anti-parallel manner to the target. Moreover, the present single-stranded circular oligonucleotides can bind to both single-stranded and double-stranded target nucleic acids. The present invention also provides methods of making and using these oligonucleotides as well as kits and pharmaceutical compositions containing these oligonucleotides.
A single-stranded circular oligonucleotide comprising at least one parallel binding (P) domain and at least one anti-parallel binding (AP) domain and further comprising at least one first loop domain and at least one second loop domain wherein said P domain and said AP domain have sufficient complementarity to bind detectably to one strand of a defined nucleic acid target, wherein said P domain is capable of binding in a parallel manner to said target, and said AP domain is capable of binding in an anti-parallel manner to said target, and wherein a first end of said P and AP domains are separated by said first loop domain and a second end of said P and AP domains are separated by said second loop domain.
A single-stranded circular oligonucleotide comprising at least one parallel binding (P) domain and at least one anti-parallel binding (AP) domain, wherein each P and AP domain has sufficient complementarity to bind detectably to one strand of a defined nucleic acid target and wherein said P domain binds in a parallel manner to said target, and a corresponding AP domain binds in an anti-parallel manner to said target.
A single-stranded circular oligonucleotide comprising a parallel binding (P) domain wherein said P domain has sufficient complementarity to bind detectably to one strand of a defined nucleic acid target in a parallel manner to said target.
A single-stranded circular oligonucleotide comprising at least one binding domain selected from the group consisting of a parallel binding (P) domain, and a Hoogstein anti-parallel (HAP) domain.
A single-stranded circular oligonucleotide comprising a Hoogsteen anti-parallel (HAP) domain wherein said HAP domain has sufficient complementarity to bind detectably to one strand of a duplex nucleic acid target in an anti-parallel manner to said target strand.
A single stranded circular oligonucleotide comprising at least two different types of binding domains selected from the group consisting of a parallel binding (P) domain, a Hoogstein anti-parallel (HAP) domain and an anti-parallel binding (AP) domain and having loop domains which connect the ends of the binding domains.
Dependencies are inferred from the claim text.