Nucleic acid probes to Chlamydia pneumoniae
AssigneeGen-Probe Incorporated
InventorsPhilip Hammond, Anthony Endozo
Life of this patent
Estimating the term…
Abstract
Hybridization assay probes specific for Chlamydia pneumoniae and no other Chlamydia species.
Claims47
An isolated or purified hybridization assay probe of 10 to 100 nucleotides comprising an oligonucleotide which will form a detectable hybrid with Chlamydia pneumoniae nucleic acid under selective hybridization conditions, and will not form a detectable hybrid with nucleic acid from Chlamydia psiticci under said conditions, said oligonucleotide having at least 14 out of 17 contiguous bases perfectly complementary to a variable region of Chlamydia pneumoniae rRNA or rDNA, said variable region comprising at least 10 contiguous bases of a region of Chlamydia pneumoniae corresponding to an Escherichia coil rRNA nucleotide base region selected from the group consisting of:
- a) bases 175-188 of 16S rRNA,
- b) bases 224-247 of 16S rRNA,
- c) bases 623-647 of 16S rRNA,
- d) bases 1008-1030 of 16S rRNA,
- e) bases 1711-1733 of 23S rRNA,
- and the nucleotide sequences perfectly complementary thereto.
A method for detecting the presence of Chlamydia pneumoniae in a sample comprising the steps of:
- a) providing to said sample or nucleic acid obtained from said sample, under selective hybridization assay conditions, a hybridization assay probe of 10 to 100 bases comprising an oligonucleotide having at least 14 out of 17 contiguous bases perfectly complementary to a variable region of Chlamydia pneumoniae rRNA or rDNA, said variable region consisting of at least 10 contiguous bases of a nucleic acid region selected from the group consisting of:
- (SEQ ID NO:
- 16) 5' CCGAATGTAGTGTAATTAGGC 3',
- (SEQ ID NO:
- 17) 5' GAGGAAGAGTTTATGCGATATCAG 3',
- (SEQ ID NO:
- 18) 5' CCCCAAGTCAGCATTTAAAACTATC 3',
- (SEQ ID NO:
- 19) 5' CTGTAGAAATACAGCTTTCCGC 3',
- (SEQ ID NO:
- 20) 5' CTTAAGGTGATTACCCAGCG 3',
- (SEQ ID NO:
- 21) 5' GCCUAAUUACACUACAUUCGG 3',
- (SEQ ID NO:
- 22) 5' CUGAUAUCGCAUAAACUCLFUCCUC 3',
- (SEQ ID NO:
- 23) 5' GAUAGUUUUAAAUGCUGACUUGGGG 3',
- (SEQ ID NO:
- 24) 5' GCGGAAAGCUGUAUUUCUACAG 3',
- (SEQ ID NO:
- 25) 5' CGCUGGGUAAUCACCUUAAG 3',
- (SEQ ID NO:
- 26) 5' CCGAAUGUAGUGUAAUUAGGC 3',
- (SEQ ID NO:
- 27) 5' GAGGAAGAGUUUAUGCGAUAUCAG 3',
- (SEQ ID NO:
- 28) 5' CCCCAAGUCAGCAUUUAAAACUAUC 3',
- (SEQ ID NO:
- 29) 5' CUGUAGAAAUACAGCUUUCCGC 3', and
- (SEQ ID NO:
- 30) 5' CUUAAGGUGAUUACCCAGCG 3',
- wherein, under selective hybridization assay conditions, said hybridization assay probe hybridizes to nucleic acid from Chlamydia pneumoniae and does not hybridize to nucleic acid from Chlamydia psiticci;
- and
- b) detecting the presence of hybrids comprised of said probe and nucleic acid of Chlamydia pneumoniae as an indication of the presence of Chlamydia pneumoniae in said sample.
Dependencies are inferred from the claim text.