Process for producing a targeted gene

A process for producing a reconstituted Sendai viral envelope comprising the F-protein, a heterologous nucleic acid and being free from contamination by other proteins, said process comprising:

• (a) adding a reducing agent to Sendai virus to reduce the HN protein and to produce a reduced virus;
• (b) subjecting the reduced virus to the step of dialysis to remove the reducing agent;
• (c) solubilizing the reduced virus with a solubilizing agent to obtain a solution;
• (d) centrifuging the solution to separate insolubles consisting of reduced HN protein and the core of said virus;
• (e) adding said nucleic acid to the supernatant;
• and
• (f) slowly removing the solubilizing agent which leads to the formation of said reconstituted Sendai viral envelope.

A process for producing a reconstituted Sendai viral envelope comprising the F-protein, a heterologous nucleic acid and being free from contamination by other proteins, said process comprising:

• (a) adding DTT to Sendai virus to reduce the HN protein and to produce a reduced virus;
• (b) subjecting the reduced virus to the step of dialysis to remove the DTT;
• (c) solubilizing the reduced virus with a solubilizing agent to obtain a solution;
• (d) centrifuging the solution to separate insolubles consisting of reduced HN protein and the core of said virus;
• (e) adding 75 micrograms of said nucleic acid per milligram of virus to the supernatant;
• and
• (f) slowly removing the solubilizing agent with a nonpolar polystyrene adsorbent, wherein the ratio of solubilizing agent to adsorbent is 1:8 which leads to the formation of said reconstituted Sendai viral envelope.