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US 5,683,864 AGranted
Combinations of hepatitis C virus (HCV) antigens for use in immunoassays for anti-HCV antibodies
AssigneeChiron Corporation
InventorsMichael Houghton, Qui-Lim Choo, George Kuo
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Abstract
Combinations of HCV antigens that have a broader range of immunological reactivity than any single HCV antigen. The combinations consist of an antigen from the C domain of the HCV polyprotein, and at least one additional HCV antigen from either the NS3 domain, the NS4 domain, the S domain, or the NS5 domain, and are in the form of a fusion protein, a simple physical mixture, or the individual antigens commonly bound to a solid matrix.
Claims22
A combination of hepatitis C viral (HCV) antigens comprising:
- (a) a first HCV antigen comprising the C domain or an immunologically reactive fragment thereof containing at least 8 amino acids, said C domain consisting of amino acids 1 to 120 of the HCV polyprotein;
- and
- (b) two additional HCV antigens from two different HCV polyprotein domains each independently selected from the group consisting of
- (i) an HCV antigen comprising the NS3 domain or an immunologically reactive fragment thereof containing at least 8 amino acids, said NS3 domain consisting of amino acids 1050 to 1640 of the HCV polyprotein;
- (ii) an HCV antigen comprising the NS4 domain or an immunologically reactive fragment thereof containing at least 8 amino acids, said NS4 domain consisting of amino acids 1640 to 2000 of the HCV polyprotein;
- (iii) an HCV antigen comprising the S domain or an immunologically reactive fragment thereof containing at least 8 amino acids, said S domain consisting of amino acids 120 to 400 of the HCV polyprotein;
- and
- (iv) an HCV antigen comprising the NS5 domain or an immunologically reactive fragment thereof containing at least 8 amino acids, said NS5 domain consisting of amino acids 2000 to 3011 of the HCV polyprotein.
5 more elements
A combination of hepatitis C vital (HCV) antigens comprising:
- (a) a first HCV antigen which is amino acids 1-122 of the HCV polyprotein or an immunologically reactive fragment thereof containing at least 8 amino acids;
- and
- (b) two additional HCV antigens from two different HCV polyprotein domains each independently selected from the group consisting of
- (i) an HCV antigen comprising the NS3 domain or an immunologically reactive fragment thereof containing at least 8 amino acids, said NS3 domain consisting of amino acids 1050 to 1640 of the HCV polyprotein;
- (ii) an HCV antigen comprising the NS4 domain or an immunologically reactive fragment thereof containing at least 8 amino acids, said NS4 domain consisting of amino acids 1640 to 2000 of the HCV polyprotein:
- (iii) an HCV antigen comprising the S domain or an immunologically reactive fragment thereof containing at least 8 amino acids, said S domain consisting of amino acids 120 to 400 of the HCV polyprotein;
- and
- (iv) an HCV antigen comprising the NS5 domain or an immunologically reactive fragment thereof containing at least 8 amino acids, said NS5 domain consisting of amino acids 2000 to 3011 of the HCV polyprotein.
5 more elements
A combination of hepatitis C viral (HCV) antigens comprising:
- (a) a first HCV antigen which is amino acids 9-177 of the HCV polyprotein or an immunologically reactive fragment thereof containing at least 8 amino acids;
- and
- (b) two additional HCV antigens from two different HCV polyprotein domains each independently selected from the group consisting of
- (i) an HCV antigen comprising the NS3 domain or an immunologically reactive fragment thereof containing at least 8 amino acids, said NS3 domain consisting of amino acids 1050 to 1640 of the HCV polyprotein;
- (ii) an HCV antigen comprising the NS4 domain or an immunologically reactive fragment thereof containing at least 8 amino acids, said NS4 domain consisting of amino acids 1640 to 2000 of the HCV polyprotein;
- (iii) an HCV antigen comprising the S domain or an immunologically reactive fragment thereof containing at least 8 amino acids, said S domain consisting of amino acids 120 to 400 of the HCV polyprotein;
- and
- (iv) an HCV antigen comprising the NS5 domain or an immunologically reactive fragment thereof containing at least 8 amino acids, said NS5 domain consisting of amino acids 2000 to 3011 of the HCV polyprotein.
5 more elements
Dependencies are inferred from the claim text.