Unglycosylated recombinant human lymphotoxin polypeptides and compositions
AssigneeGenentech, Inc.
InventorsBharat B. Aggarwal, Patrick W. Gray, Glenn E. Nedwin
Life of this patent
Estimating the term…
Abstract
Biologically active lymphotoxin polypeptides are synthesized in recombinant cell culture. Novel nucleic acid and vectors incorporating same are provided. The compositions and processes herein enable the economical preparation of compositions containing uniform lymphotoxin polypeptides and variant lymphotoxins having amino acid sequences that differ from those found in nature. The lymphotoxins are purified to a specific activity of 2-10.times.10.sup.7 units/mg of protein by purification using a novel immobilized, lymphotoxin-neutralizing monoclonal antibody.
Claims23
A substantially homogeneous, biologically active human lymphotoxin species comprising at least residues His+24 to Leu+171 of the amino acid sequence of FIGS. 2A-I and 2A-II, which is unglycosylated.
A substantially homogeneous, biologically active lymphotoxin species consisting of residues His+24 to Leu+171 of the amino acid sequence of FIGS. 2A-I and 2A-II, which is unglycosylated.
A substantially homogeneous, biologically active lymphotoxin species consisting of residues Leu+1 to Leu+171 of the amino acid sequence of FIGS. 2A-I and 2A-II, which is unglycosylated.
Dependencies are inferred from the claim text.