Antibody against human mucin core protein and method of preparing and using same
AssigneeImperial Cancer Research Technology, Ltd.
InventorsJoyce Taylor-Papadimitriou, Sandra Gendler, Joy Burchell
Life of this patent
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Abstract
Antibodies which bind to the core protein of the human polymorphic epithelial mucin glycoprotein are useful in the diagnosis of cancer.
Claims18
An antibody, or an epitope-binding fragment of an antibody, which binds to a peptide epitope of the core protein of human mammary polymorphic epithelial mucin glycoprotein, wherein said core protein is that which is bound by the reference antibody SM3, secreted by hybridoma cell line HSM3 (accession number ECACC 87010701), and wherein said antibody or fragment binds to said glycoprotein as secreted by human mammary epithelial tumor cells and does not bind to said glycoprotein as secreted by the normal lactating human mammary gland.
An antibody, or an epitope-binding fragment of an antibody, which binds to a peptide epitope of the core protein of the human mammary polymorphic epithelial mucin glycoprotein, which epitope is also bound by the reference antibody SM3, secreted by the hybridoma cell line HSM3, deposited with the European Collection of Animal Cell Cultures under accession number ECACC 87010701.
A method of diagnosing cancer in a human or animal, which comprises the steps of:
- (a) administering to a human or animal, or to a sample obtained from a human or animal, a diagnostically effective amount of an antibody or an epitope-binding fragment thereof, which antibody or fragment binds to an epitope of the core protein of human mammary polymorphic epithelial mucin glycoprotein, which core protein is also bound by the reference antibody, SM3, secreted by hybridoma cell line HSM3, which hybridoma has the accession number ECACC 87010701, and wherein said antibody or fragment binds to said glycoprotein as secreted by human mammary epithelial rumor cells and does not bind to said glycoprotein as secreted by the normal lactating human mammary gland;
- (b) determining whether said antibody or fragment is bound to said epitope;
- and
- (c) diagnosing the presence or absence of cancer as a result of said determination.
A method of diagnosing cancer in a human or animal which comprises the steps of:
- (a) administering to a human or animal, or to a sample obtained from a human or animal, a diagnostically effective amount of an antibody or an epitope-binding fragment thereof, wherein said antibody or fragment binds to an epitope of the core protein of human mammary polymorphic epithelial mucin glycoprotein, which epitope is also bound by the reference antibody SM3, secreted by the hybridoma cell line HSM3, deposited with the European Collection of Animal Cell Cultures under accession number ECACC 87010701;
- and
- (b) determining whether said antibody or fragment is bound to said epitope;
- and
- (c) diagnosing the presence or absence of cancer as a result of said determination.
Dependencies are inferred from the claim text.