US 5,682,884 AGranted

Strip electrode with screen printing

AssigneeMedisense, Inc.

InventorsHugh Allen Oliver Hill, Irving John Higgins, James Michael McCann, Graham Davis

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Abstract

A sensor system that detects a current representative of a compound in a liquid mixture features a two-electrode strip adapted for releasable attachment to signal readout circuitry. The strip comprises an elongated support (preferably flat) adapted for releasable attachment to the readout circuitry; a first conductor and a second conductor each extend along the support and comprise means for connection to the circuitry. An active electrode, positioned to contact the liquid mixture and the first conductor, comprises a deposit of an enzyme capable of catalyzing a reaction involving the compound and (preferably) an electron mediator, capable of transferring electrons between the enzyme-catalyzed reaction and the first conductor. A reference electrode is positioned to contact the mixture and the second conductor. The system includes circuitry adapted to provide an electrical signal representative of the current. The two-electrode strip is manufactured, e.g., by screen printing the enzyme (and preferably the mediator) onto the substrate.

Claims17

Dependent claims

A single use disposable electrode strip for attachment to signal readout circuitry of a sensor system to detect a current representative of a compound in a blood sample, the strip comprising:

a) an elongated support having a substantially flat, planar surface, adapted for releasable attachment to said readout circuitry;
b) a first conductor extending along said surface and comprising a conductive element for connection to said readout circuitry;
c) an active electrode on said surface in contact with said first conductor, said active electrode comprising, in a single layer on said surface an admixture of an enzyme capable of catalyzing a reaction involving a substrate for said enzyme, a conductive material, and a mediator capable of transferring electrons transferred between said enzyme-catalyzed reaction and said first conductor to create a current representative of the activity of said enzyme and representative of said compound;
d) a second conductor extending along said surface, comprising a conductive element for connection to said readout circuitry;
and
e) a reference electrode in contact with said second conductor,
said first conductor and said active electrode spaced apart from said second conductor and said reference electrode and said active electrode being sized and positioned to present a combined effective area small enough to be completely coverable by a drop of blood contacting on the active electrode, whereby said blood completes an electrical circuit across said active electrode and said reference electrode, for amperometric detection of said activity of said enzyme.

4 more elements

Apparatus for detecting a current representative of a compound in a blood sample, the apparatus comprising signal readout circuitry and an electrode strip comprising:

a) an elongated support having a substantially flat, planar surface, adapted for releasable attachment to said signal-readout circuitry;
b) a first conductor extending along said surface and comprising a conductive element for connection to said readout circuitry;
c) an active electrode on said surface in contact with said first conductor, said active electrode comprising, in a single layer on said surface, an admixture of an enzyme capable of catalyzing a reaction involving a substrate for said enzyme, a conductive material, and a mediator capable of transferring electrons transferred between said enzyme-catalyzed reaction and said first conductor to create a current representative of the activity of said enzyme and representative of said compound;
d) a second conductor extending along said surface, comprising a conductive element for connection to said readout circuitry;
and
e) a reference electrode in contact with said second conductive conductor,
said first conductor and said active electrode spaced apart from said second conductor and said reference electrode, said electrodes being sized and positioned sufficiently close together to present a combined effective area small enough to be completely coverable by a drop of blood contacting on said active electrode, whereby said blood completes an electrical circuit across said active electrode and said reference electrode, for amperometric detection of said activity of said enzyme.

4 more elements

Dependencies are inferred from the claim text.

Facts

Application: 08/281,131Filed 27 Jul 1994 · continuation
Priority: GB 83122615 May 1983
Granted: 4 Nov 1997
Term ends: Not estimatedThe source records don’t support an estimate.

Classification11 codes

A61B 5/1486
Using chemical or electrochemical methods, e.g. by polarographic means: using enzyme electrodes, e.g. with immobilised oxidase
A61B 5/14865
Using enzyme electrodes, e.g. with immobilised oxidase: invasive, e.g. introduced into the body by a catheter or needle or using implanted sensors
A61B 5/6848
Mounted on an invasive device: needles
C12Q 1/001
Measuring or testing processes involving enzymes, nucleic acids or microorganisms ; Compositions therefor; Processes of preparing such…

Family

Serial607,599Parent of this continuation-in-part · filed 7 May 1984
Serial02,120Parent of this continuation · filed 12 Jan 1987
Serial634,968Parent of this continuation · filed 7 Jan 1991
Serial768,359Parent of this continuation · filed 30 Sep 1991
Serial888,264Parent of this continuation · filed 22 May 1992
US 5,682,884 AThis patent

From the US filing history. Foreign family members aren’t listed.