Process for the isolation and purification of isoflavones
AssigneeHauser, Inc.
InventorsBoLin Zheng, John A. Yegge, David T. Bailey, James L. Sullivan
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Abstract
The present invention relates to a process for the isolation and purification of isoflavones from a number of different biomass sources. More particularly, the present invention relates to a three-step process whereby a biomass containing isoflavones with a solvent thereby forming an extract that is subsequently fractionated using a reverse phase matrix in combination with a step gradient elution, wherein the resulting fractions eluted from the column contain specific isoflavones that are later crystallized. The purified isoflavone glycosides may then be hydrolyzed to their respective aglycone.
Claims35
A method of isolating isoflavones from any plant material that contains one or more type of isoflavone compounds, comprising the steps of:
- contacting the plant material for a selected period of time with a first solvent whereby at least some of said isoflavone compounds are soluble and transported into said first solvent thereby forming a crude extract;
- passing said crude extract through a column wherein said column contains an absorbent for specifically adsorbing said isoflavone compounds;
- desorbing said isoflavone compounds sequentially from said adsorbent by flowing a series of eluant mixtures making up a step gradient elution over said column;
- and
- collecting each of the individual eluant mixtures of said step gradient elution flowing through said column wherein each of said individual eluant mixture contains primarily isoflavone compound.
A method of isolating isoflavones from any plant material that contains one or more isoflavone compounds, comprising the steps of:
- contacting the plant material for a selected period of time with a first solvent whereby at least some of said isoflavone compounds are soluble or transported into said first solvent thereby forming a crude extract;
- passing said crude extract having one or more of said isoflavone compounds through a low pressure column wherein said low pressure column contains an adsorbent for specifically adsorbing said isoflavone compounds;
- desorbing said isoflavone compounds one at a time from said adsorbent by sequentially flowing a series of eluants over said adsorbent, wherein each eluant in said series comprises a specific ratio of a second solvent to water that specifically desorbs and transports at least one isoflavone compound;
- and
- collecting separately, each of said eluants transporting a specific isoflavone compound.
A method of isolating isoflavones from any plant material that contains one or more isoflavone compounds, comprising the steps of:
- extracting one or more isoflavone compounds from the plant material;
- adsorbing said isoflavone compounds to a reverse phase matrix;
- desorbing a first isoflavone compound from said reverse phase matrix by flowing a first eluant having a ratio of a solvent to water over said matrix;
- collecting said first eluant after said first eluant has flowed over said matrix;
- desorbing a second isoflavone compound from said reverse phase matrix by flowing a second eluant, having a ratio of said solvent to water which is higher than the ratio of said solvent to water making up said first eluant, over said matrix;
- and
- collecting said second eluant after said second eluant has flowed over said matrix.
Dependencies are inferred from the claim text.